摘要
目的:探讨芒果苷对链脲佐菌素诱导的糖尿病大鼠心肌的保护作用。方法:将SD大鼠随机分为5组,即正常对照组、糖尿病模型组、芒果苷低、中、高剂量组,链脲佐菌素法腹腔注射建立糖尿病大鼠模型,检测各组血糖、血及心肌组织丙二醛(malondialchehyche,MDA)的含量、超氧化物歧化酶(SOD)的活性、心肌羟脯氨酸含量,Western blot方法检测心肌β-catenin蛋白表达;HE染色光镜观察心肌组织形态改变。结果:与糖尿病模型组相比较,芒果苷治疗组大鼠空腹血糖水平明显降低,血清及心肌组织SOD活性升高、MDA含量显著降低,且心肌羟脯氨酸含量有所下降;治疗组大鼠心肌β-catenin蛋白表达较糖尿病模型组明显下降。结论:芒果苷能够减轻链脲佐菌素诱导的糖尿病大鼠血清及心肌氧化应激反应,降低心肌β-catenin蛋白表达水平,对糖尿病心肌具有一定的保护作用。
AIM： To investigate the protective effect of mangiferin on myocardium of diabetic rats.METHODS： Male Sprague-Dawley rats were randomly divided into 5 groups： normal control group,diabetic model group,high-dose,medium-dose and low-dose mangiferin groups. Streptozotocin was injected intraperitoneally to establish diabetic model.Fasting plasma glucose levels,activities of superoxide dismutase（ SOD）, content of malonaldehyde（ MDA） in serum and myocardium and myocardial hydroxyproline were detected. Western blot was used to test the expression of β-catenin protein in myocardium. The morphological changes of myocardium were inspected through microscopy. RESULTS： Compared with diabetic model group,mangiferin significantly reduced the level of fasting plasma glucose, the content of MDA, myocardial hydroxyproline and increased the activities of SOD in serum and myocardium. The levels of β-catenin in myocardium were dramatically decreased in rats treated with mangiferin compared with diabetic model group. CONCLUSION： Mangiferin reduces the degree of oxidative stress and inhibits the expression ofβ-catenin in myocardium of diabetic rats induced by STZ. It may have a protective effect on myocardium of diabetic rats.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2016年第8期859-862,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
安徽省高等学校省级自然科学研究项目(KJ2013B315)
安徽省高校优秀青年人才支持计划重点项目(gxyq ZD2016172)
