摘要
采取粉末活性炭吸附-双水相萃取的工艺方法,以巢湖新鲜蓝藻藻泥为原料提纯藻蓝蛋白,在考虑煤质粉末活性炭添加量的基础上,探究粉末活性炭纯化藻蓝蛋白的效果。通过单因素和正交试验,确定粉末活性炭吸附-双水相萃取法纯化藻蓝蛋白的最优工艺条件:PEG分子量为4 000做高聚物,PEG浓度为4%,磷酸钾盐浓度为16%,体系p H为7.0。验证试验结果表明,经过优化工艺纯化后藻蓝蛋白纯度可达到3.461 7,组合工艺回收率为63%。与传统方法相比,该工艺具有设备小、成本低、周期短等特点。
Extraction and purification of C-phycocyanin were performed by the combined use of powdered activated carbon（ PAC） treatment and aqueous two-phase extraction from fresh Cyanobacteria mud in Chaohu Lake. Firstly,the effects of PAC on purification of C-phycocyanin were studied by considering the dosage of powdered activated carbon. Then,the single factor and orthogonal experiments were conducted to determine the optimum conditions in the PAC treatment and aqueous two-phase purification, which were PEG 4000 as polymer, the PEG 4000 concentration 4%,the potassium phosphate concentration 16% and p H 7. 0. The verification test showed that after the purification by the optimized process,the purity and recovery rate of C-phycocyanin could reach to 3. 461 7 and63%,respectively. It was with less equipment requirements,lower cost and shorter time,etc.,compared with traditional methods.
出处
《环境工程技术学报》
CAS
2016年第5期469-475,共7页
Journal of Environmental Engineering Technology
基金
国家水体污染控制与治理科技重大专项(2012ZX07103-004)
关键词
粉末活性炭
双水相
提取纯化
藻蓝蛋白
powdered activated carbon
aqueous two-phase extraction
extraction and purification
C-phycocyanin
