摘要
试验旨在检测水牛瘦素(Leptin)基因序列的多态性,为进一步开展水牛标记辅助育种研究奠定基础。运用DNA混合池结合直接测序及高分辨熔解曲线(HRM)法在182头奶水牛个体中进行Leptin基因SNP位点的筛选和分型。结果表明,在试验群体中Leptin基因共发现7个SNPs,位于内含子1、内含子3和外显子3区域,分别是A3123G、A3776G、A4154G、A5228G、A5524C、G5573T和A5751C。除了SNP5和SNP6位点在尼里-拉菲水牛群体中的多态信息含量(PIC)属于低度多态之外,所有SNPs位点在3个水牛群体中均属于中度多态。经χ2检验,所有SNPs位点的突变在尼里-拉菲水牛群体均达到Hardy-Weinberg平衡状态(P>0.05)。本研究成功筛查7个水牛Leptin基因SNPs位点并进行了基因分型,为下一步开展奶水牛Leptin基因的标记-性状关联分析奠定基础。
This study was aimed to detect the polymorphisms of Leptin gene in buffalo that provided a fundamental for further study on marker assisted breeding in buffaloes.The single nuclease polymorphisms（SNP）of Leptingene were identified and genotyped by using DNA pooled sequencing and high-resolution melting（HRM）method in three buffalo breeds with 182 buffalo individuals,respectively.The results showed that seven SNPs of Leptin gene were identified in studied population that were located in the intron 1,intron 2and exon 3regions,respectively.All the SNPs loci were moderate polymorphism except for the SNP5 and SNP6.Theχ2-test indicated that all the SNPs loci were in agreement with Hardy-Weinberg equilibrium in Nili-Ravi population（P0.05）.Our findings revealed that seven SNPs of Leptin gene in buffalo were identified and genotyped in population,which provide the data support for further analyzing the associations between these polymorphism and production traits in buffaloes.
出处
《中国畜牧兽医》
CAS
北大核心
2016年第9期2418-2424,共7页
China Animal Husbandry & Veterinary Medicine
基金
广西科技攻关国际交流与合作项目(桂科合14123001-5)
广西水产畜牧兽医局科技项目(桂渔牧科201452032)
广西自然科学基金重点项目(2014GXNSFDA118017)
