摘要
目的建立HPLC测定白花蛇舌草中熊果酸、异熊果酸和对香豆酸的含量。方法采用Diamonsil C18 ODS（4.6 mm×250 mm,5μm）,流动相为乙腈-15 mmol·L？1 NH4Ac（12∶88,p H 3.5）,流速为1.0 m L·min？1,检测波长为308 nm,柱温为35℃。结果熊果酸、异熊果酸和对香豆酸线性范围分别为0.075 7~2.271μg（R2=0.999 6）,0.010 3~0.309μg（R2=0.999 2）和0.020 2~0.606μg（R2=0.999 6）;3种成分测定的平均回收率分别为99.9%（RSD=0.7%）,99.0%（RSD=1.7%）和101.1%（RSD=0.7%）。结论本法简便、准确度高、稳定性和重复性好,可用于白花蛇舌草中指标性成分定量分析和质量控制。
OBJECTIVE To establish an HPLC method for the simultaneous determination of ursolic acid, isoursolic acid and p-coumaric acid in Hedyotis diffusa Willd.. METHODS Diamonsil C18 ODS（4.6 mm×250 mm, 5 μm） was used; mobile phase was acetonitrile and 15 mmol·L？1 NH4AC（12∶88, p H3.5）; flow rate： 1.0 m L·min？1; wavelength： 308 nm; column temperature： 35 ℃. RESULTS Three ingredients showed good linear relationships, ursolic acid： 0.075 7？2.271 μg（R2=0.999 6）, isoursolic acid： 0.010 3？0.309 μg（R2=0.999 2） and p-coumaric acid： 0.020 2？0.606 μg（R2=0.999 6）, with the mean recoveries as 99.9%（RSD=0.7%）, 99.0%（RSD=1.7%） and 101.1%（RSD=0.7%）, respectively. CONCLUSION This method is simple with high precision, repeatability, and stability, so it can be used to quantitative analysis and quality control of Hedyotis diffusa Willd.
出处
《中国现代应用药学》
CAS
CSCD
2016年第8期1045-1048,共4页
Chinese Journal of Modern Applied Pharmacy
