摘要
目的采用HPLC法测定复方北豆根氨酚那敏片中咖啡因、马来酸氯苯那敏与对乙酰氨基酚的含量,并检测咖啡因与马来酸氯苯那敏的含量均匀度。方法采用C_(18)色谱柱(250 mm×4.6 mm,5μm),以p H4.0磷酸二氢钾溶液-甲醇为流动相,梯度洗脱,检测波长为225 nm(咖啡因与马来酸氯苯那敏)、254 nm(对乙酰氨基酚),流速1.0 m L·min^(-1),柱温30℃,进样量20μL。结果咖啡因、马来酸氯苯那敏和对乙酰氨基酚浓度的线性范围分别为72.41~217.2、14.81~44.44、31.06~93.18μg·m L^(-1),平均回收率分别为100.58%、99.18%、99.64%(n=9)。结论所用法简便、准确,可同时测定多种组分,可用于该制剂的质量控制。
OBJECTIVE To determine the content of caffeine, ehlorphenarnine maleate and acetaminophen in Compound asiatic moonseed ,paracetamol and ehlophenamine maleate tablets（CAPCT） by HPLC and carry out the content uniformity test for caffeine and ehlorphenamine maleat. METHODS The HPLC method was performed on Hibar C18 column （250 mm× 4.6 mm,5 μm）. The mobile phase was potassium dihydrogen phosphate solution（pH4.0） methanol with gradient elution. The flow rate was 1.0 mL·min^- 1. The detection wave length was 225 nm for caffeine and ehlorphenamine maleate, and 254 nm for acetaminophen. The temperature of column was 30 ℃. The injection volume was 20 μL. RESULTS The calibration curves were liner in the ranges of 72.41 -217.2 μg·mL^-1 , 14.81 -44.44μg·mL^-1 ,31.06 - 93.18 μg·mL^-1 for the three components, respectively. The average recoveries were 100. 58% , 99. 18% and 99.64% （n = 9） ,respectively. CONCLUSION The method is convenient and accurate can simultaneously determine a variety of ingredients and can be used for the quality control of CAPCT.
出处
《华西药学杂志》
CAS
CSCD
2016年第4期412-414,共3页
West China Journal of Pharmaceutical Sciences
关键词
高效液相色谱法
复方北豆根氨酚那敏片
咖啡因
马来酸氯苯那敏
对乙酰氨基酚
含量测定
定量控制
HPLC
Compound asiatic moonseed, paracetamol and ehlophenamine maleate tablets
Caffeine
Chlorphenamine maleate
Aeetaminophen
Content determination
Quality control
