摘要
目的:探讨黄芪多糖对胃癌前病变(PLGC)模型大鼠胃黏膜P53、P65、血管内皮生长因子(VEGF)蛋白及细胞凋亡指数(AI)的影响。方法:将60只大鼠随机分为正常对照组、模型对照组、维酶素片组(阳性药物,0.01 g/kg)和黄芪多糖低、高剂量组(0.5、1.0 g/kg),除正常对照组外,其余各组复制PLGC模型。建模成功后,各给药组大鼠ig相应药物,正常对照组和模型对照组大鼠ig生理盐水,每天1次,连续8周。检测各组大鼠胃黏膜P53、P65、VEGF蛋白的表达及AI,并观察胃黏膜病理变化。结果:与正常对照组比较,模型对照组及各给药组大鼠胃黏膜P53、P65、VEGF蛋白表达增强(P<0.05),AI降低;胃黏膜有变薄、欠光滑、黏液减少等PLGC现象。与模型对照组比较,各给药组大鼠胃黏膜P53、P65、VEGF蛋白表达减弱、AI升高,且黄芪多糖低、高剂量组上述指标变化较维酶素片组更为明显(P<0.05);胃黏膜病理情况得到改善。与黄芪多糖低剂量组比较,黄芪多糖高剂量组大鼠胃黏膜P53、P65、VEGF蛋白表达减弱、AI升高更为明显(P<0.05)。结论:黄芪多糖可下调PLGC模型大鼠P53、P65、VEGF蛋白表达,降低AI,从而控制PLGC进展。
OBJECTIVE：To investigate the effects of astragalus polysaccharides on the protein expression of P53,P65 and vascular endothelial growth factor（VEGF） and apoptosis index（AI） in rats with precancerous lesions of gastric cancer（PLGC）.METHODS：60 rats were randomly divided into normal control group,model control group,Vitacoenzyme tablet group（positive drug,0.01 g/kg）,astragalus polysaccharide low-dose and high-dose groups（0.5,1.0 g/kg）. Except for normal control group,PLGC model was induced in those groups. After modeling,those groups were given relevant medicine intragastrically,and normal control group and model control group were given normal saline intragastrically,once a day,for 8 weeks. The protein expression of P53,P65,VEGF in gastric mucosa and AI of rats were determined,and the pathological changes of gastric mucosa was observed. RESULTS：Compared with normal control group,the protein expression of P53,P65,VEGF in gastric mucosa increased in model control group and treatment groups（P〈0.05）,while AI decreased;gastric mucosal lesions occurred like thin,less smooth,less mucus and other precancerous lesions. Compared with model control group,protein expression of P53,P65,VEGF in gastric mucosa decreased in treatment groups,while AI increased;the changes of above indexes in astragalus polysaccharide low-dose and high-dose groups were more significant than in Vitacoenzyme tablet group（P〈0.05）;the pathological changes were improved. Compared with astragalus polysaccharide low-dose group,the protein expression of P53,P65,VEGF in gastric mucosa decreased in high-dose group, while AI increased significantly（P〈0.05）. CONCLUSIONS： Astragalus polysaccharide can down-regulate the protein expression of P53,P65,VEGF in PLGC model rats and down-regulate AI so as to control the progress of PLGC.
出处
《中国药房》
CAS
北大核心
2016年第22期3069-3071,共3页
China Pharmacy
关键词
黄芪多糖
胃癌前病变
细胞凋亡
基因突变
大鼠
Astragalus polysaccharide
Precancerous lesions of gastric cancer
Apoptosis
Gene mutation
Rats
