摘要
阿魏酸是西瓜分泌的化感物质之一,在西瓜自毒作用中具有重要的作用。选用质量浓度为0~1 000 mg·L^(-1)的阿魏酸浸泡处理西瓜种子,观察西瓜种子的萌发、生长,并检测处理7 d后幼苗不同部位生理生化指标的变化。结果表明,阿魏酸对种子的萌发没有影响,但显著降低了种子活力指数,抑制了根和茎的伸长及鲜质量的增加。随着阿魏酸质量浓度的提高,根和茎中的超氧阴离子浓度及膜质过氧化程度显著增加,抗氧化酶活性过氧化氢酶(CAT)的活性也显著提高,而过氧化物酶(POD)和超氧化物歧化酶(SOD)的活性则呈现先升后降的趋势,苯丙氨酸解氨酶(PAL)活性也呈现先升后降的趋势,并导致西瓜根中酚酸类物质的质量浓度持续升高。说明西瓜幼苗抗氧化系统的激活能在一定程度上降低酚类物质的影响,阿魏酸可能通过在根中过量积累酚类物质而影响根的生长与发育。
Ferulic acid is one of allelochemicals secreted by the roots of watermelon, which plays an important role in the au-totoxicity process. In this study, ferulic acid ranging from 0 to 1 000 mg·L^（-1）was used to treat the seeds of watermelon, subse-quently, seed germination, seedling growth, as well as the cellular physiological and biochemical changes was monitored af-ter 7 days. The results showed that ferulic acid had no significant effect on seed germination, while it significantly reducedseed vigor index, inhibited the elongation of roots and shoots along with the their fresh weight. Superoxide anion radical andlipid peroxidation both in the roots and shoots increased significantly with the increased ferulic acid concentration, at thesame time, the antioxidant enzyme activities of catalase（CAT） increased accordingly, while the activities of peroxidase（POD） and superoxide dismutase（SOD） significantly increased firstly and then decreased, similar response pattern was ob-served in the activity of phenylalanine ammonia-lyase（PAL）. Consequently, the concentration of phenolic acid in watermel-on seedling roots increase steadily. These results indicates that the activation of antioxidant enzyme can reduce the injure im-posed by the phenolic compound accumulated in root in watermelon seedlings, and the effect of ferulic acid on root growthand development can be attributed to excess accumulation of phenolic compound.
出处
《中国瓜菜》
CAS
北大核心
2016年第7期14-18,共5页
China Cucurbits And Vegetables
基金
中国农业科学院科技创新工程项目(CAAS-ASTIP-2015-ZFRI)
关键词
西瓜
种子
幼苗
酚酸
化感作用
抗氧化
苯丙氨酸解氨酶
Watermelon
Seed
Seedling
Pherulic acid
Allelopathic effect
Antioxidant activity
Phenylalanine ammonia-lyase
