摘要
本文基于MiSeq高通量测序技术对南移福建池塘养殖仿刺参(Apostichopus japonicus)肠道菌落结构进行分析。结果表明,仿刺参前肠(1 C_1)、中肠(1 C_2)和后肠(1C_3)测得的分类操作单元(OTUs)分别为424、444和414;三组样品的菌群物种丰度没有较大差别,但优势菌种存在较大差异;优势菌群方面,后肠的优势菌群为Haliea属和乳球菌属(Lactococcus)(分别占后肠总菌数的11.97%和10.37%),而前肠和中肠的优势菌群均是乳球菌属和芽孢杆菌属(Bacillus),两者在前肠菌落中的占比分别为27.91%和6.08%,在中肠中的占比分别为33.24%和6.97%。在重复性方面,三组样品的菌落组成都有重叠,重叠率为74.35%,其中前肠与中肠相似度较高,菌株种类有90%以上重叠。本文研究为仿刺参肠道益生菌的开发利用提供基础资料。
The gut microbiota is essential in shaping many of its host＇s functional attributes. The gut microbiota is necessary for the proper physiological development of the gut and for the animaPs ability to digest and convert plant mass into food products. Thus based on high throughput sequencing technology MiSeq, the research aims at analyzing the structure of intestinal bacterial colonies of Apostichopus japonicus cultured in pond polyculture of Fujian. The results showed that the units of classification operation （ OTUs） of the three samples were 424, 444 and 414,respectively. The dominant strains were quite different among these samples. The hindgut dominant bacteria were Haliea （11. 97 % ） and Lactococcus （10. 3 1 % ） , while both foregut and midgufs dominance were Lactococcus and Bacillus. In addition, the percentage of Lactococcus in foregut and midgut were 27. 91% and 6. 08% , respectively, as for Bacillus were 33. 2 4 % and 6.91% . In repeatability, colonies consisting of three samples had overlapping （74. 35 % ） , which foregut and midgut had high similarity ,the strain types more than 9 0 % overlap. Therefore, the study aimed at providing basic information for the development and utilization of Apostichopus japonicu intestinal flora.
出处
《渔业研究》
2016年第3期192-201,共10页
Journal of Fisheries Research
基金
福建省科技计划项目(2014R1003-1)
福建省海洋高新产业发展专项项目(闽海洋高新[2015]29号)
闽台重要海洋生物资源高值化开发技术公共服务平台(2014FJPT01)
福建重要海洋经济生物种质库与资源高效开发技术公共服务平台(14PZY017NF17)
关键词
MiSeq高通量测序
仿刺参
池塘养殖
肠道菌群
MiSeq high - throughput sequencing
Apostichopus japonicus
pond culture
gut microbiota
