摘要
目的建立五圣伏龙胶囊定性定量方法。方法采用显微鉴别方法对处方中的山药进行鉴别,采用薄层色谱法对赤芍进行鉴别,探索研究地龙、水蛭的薄层色谱鉴别。采用反相高效液相色谱法对处方中赤芍的有效成分芍药苷进行定量研究,采用C_(18)色谱柱,以乙腈-0.1%磷酸溶液(14∶86)为流动相,检测波长为230 nm,流速为1.0 m L·min^(-1),柱温:室温。结果在显微图片中可检出山药,在薄层色谱中可检出赤芍,薄层色谱中地龙、水蛭因互相干扰未收入质量标准中,芍药苷进样量在0.060 2~1.806μg范围内线性关系良好(r=0.999 5);平均回收率为99.47%(n=9)。结论该方法简便、准确、可靠,可用于五圣伏龙胶囊的质量控制。
Objective To establish a qualitative and quantitative method of Wushengfulong Capsules. Methods Di-oscoreae rhizome were identified by microscope. Paeoniae radix rubra was identified by TLC. HPLC method was used to de-termine the effective content of paeoniflorin in Paeoniae radix rubra,The HPLC method was carried on C18 column using acetonitrile - 0. 1% phosphoric acid solution（14∶86）as mobile phase,and the detection wavelength was 230 nm,the flow rate was 1. 0 mL·min - 1 and the temperature of column was 30 ℃ . Results Dioscoreae rhizome,Paeoniae radix rubra can be identified by TLC. Paeoniflorin showed a good linear relationship in the range of 0. 060 2 ~ 1. 806 μg（r = 0. 999 5）,the average recovery was 99. 47%（n = 9）. Conclusion This method was simple,reliable,accurate,can be used to control the quality of Wushengfulong Capsules.
出处
《药学研究》
CAS
2016年第7期396-398,407,共4页
Journal of Pharmaceutical Research
