摘要
基于10年的黑土培肥定位试验,本试验采用PCR-DGGE、克隆测序技术的方法,研究了培肥处理对大豆固氮菌多样性的影响。DGGE图谱分析表明对照土壤固氮菌多样性指数最高,其次为农肥低量处理、农化1:1处理、化肥低量处理和农肥高量处理,化肥高量处理土壤固氮菌多样性指数最低。在相似度60%水平上化肥处理聚成一群,对照、农肥处理和农化1:1处理量聚成一群。培肥处理降低了土壤固氮菌的多样性,影响了土壤中固氮菌的群落结构,其中培肥高量处理比低量处理降低土壤固氮菌多样性的效果更显著。
Through a 10-year experiment site on black soil, our paper studied the effects of location fertilization managements on the nitrogen-fixing bacteria diversities of soybean in Dong-bei black soil by PCR-DGGE and clone sequencing methods. DGGE atlas analysis demonstrated that nitrogen-fixing bacteria diversities of CK was the highest and the lowest nitrogen-fixing bacteria diversities was high chemical fertilizer treatment and then was low manure fertilizer, 1/2 manure fertilizer plus 1/2 chemical fertilizer,low chemical fertilizer, and high manure fertilizer. There were two groups in similarity 60% level, one was chemical fertilizer, the other was CK, manure fertilizer, and 1/2 manure fertilizer plus1/2 chemical fertilizer. Fertilizer managements reduced nitrogen-fixing bacteria diversities of soybean and influenced community structures of nitrogen-fixing bacteria, especially high fertilizer managements could decrease nitrogen-fixing bacteria diversities of soybean more notablly than low fertilizer managements.
出处
《国土与自然资源研究》
2016年第2期67-69,共3页
Territory & Natural Resources Study
基金
黑龙江省博士后科研启动金"高效复合菌剂降解秸秆还田对土壤碳库影响机制的研究"(LBH-Q13020)
关键词
农肥
化肥
大豆固氮菌
NIFH
PCR-DGGE
organic manure
chemical fertilizer
soybean nitrogen-fixing bacteria
nif H
PCR-DGGE
