摘要
本研究对202份大白菜自然群体材料进行简化基因组测序（Specific Locus Amplified Fragment Sequencing,SLAF）,并通过测序深度、SLAF标签的多态性与参考基因组的相似程度等指标的筛选,获得了全基因组均匀分布的960个SLAF分子标记。结合相应材料的霜霉病病情调查结果,利用所述960个SLAF标记进行全基因组关联分析。结果显示：（1）在A01染色体上检测到1个新的与抗霜霉病关联的SLAF标记,即SLAFMarker A0124655323;（2）根据SLAFMarker A0124655323左右两翼最近标记之间的距离确定此热点区间为A01染色体24573724~24755150的物理范围;（3）利用该区间内的一个SNP开发出适用于KASP分型技术的SNP分子标记,在各亚群选择准确率均在80%以上;（4）在此区间找到抗病相关基因13个。本研究获得的新的抗病位点和开发的SNP分子标记,将有助于大白菜抗霜霉病分子育种。
A total of 202 Chinese cabbage（Brassica rapa ssp.pekinensis） inbred lines were used for downy mildew（DM） resistance analysis.Lines were genotyped by Specific Locus Amplified Fragment Sequencing（SLAF） markers detected by the SLAF-Seq approach,and finally 960 markers were identified and selected for Genome Wide Association Studies（GWAS）.Our major results were as follows：（1） A single SLAF locus,SLAFMarker A0124655323,on chromosome A01 were significantly associated with downy mildew resistance;（2） Based on the two nearest SLAF markers that flanking both sides of SLAFMarker A0124655323,the candidate physical interval was limited to A01：24573724~24755150;（3） A single nucleotide polymorphism（SNP） was converted into KASP system applicable marker,and the accuracy of selection in the each subgroup of natural population was above 80%;（4） A total of 13 genes was annotated as candidate disease-related genes.The newfound disease resistance loci and SNP marker will be useful in Chinese cabbage（B.rapa） breeding for improving resistance to downy mildew（DM）.
出处
《植物生理学报》
CAS
CSCD
北大核心
2016年第5期693-702,共10页
Plant Physiology Journal
基金
国家自然科学基金青年科学基金项目(31401875)~~
关键词
大白菜
霜霉病
全基因组关联分析
简化基因组测序
竞争性等位基因特异性PCR
Chinese cabbage
downy mildew
Genome-Wide Association Studies
Specific Locus Amplified Fragment Sequencing
competitive allele specific PCR
