摘要
目的 建立超高效液相色谱法（UPLC）同时测定白花蛇舌草及其混淆品中胞苷、尿苷、腺嘌呤、胸苷和腺苷5 种抗肿瘤核苷类成分的方法,并比较其含量差异.方法 采用UPLC BEH C18（2.1 mm×50 mm,1.7 μm）色谱柱,流动相为乙腈-水,梯度洗脱,检测波长254 nm,流速0.5 mL/min,柱温30 ℃.结果 5 种核苷类成分线性关系、精密度、稳定性、重复性均符合方法学相关要求,加样回收率为98.7%～101.5%.应用所建方法测定了不同产地白花蛇舌草和其常见混淆品中5 种核苷类成分的含量.结论 本研究所建立的方法简单、快速、准确、可靠,适用于白花蛇舌草及其混淆品中核苷类成分的含量测定.
Objective To explore an UPLC method for simultaneous content determination of the five nucleosides (cytidine, uridine, adenine, thymidine and adenosine) in Hedyotis diffusa and its adulterants; To compare the content differences.Methods The analysis was performed on a BEH C18 column (2.1 mm×50 mm, 1.7 μm) by UPLC eluted with acetonitrile and water in gradient mode. The flow rate was 0.5 mL/min; the detection wavelength was set at 254 nm; the column temperature was set at 30℃.Results Five nucleosides have good linear relationship, precision, stability, and repeatability according to the requirements of the methodology determination. The recoveries were among 98.7%–101.5%. Five nucleosides in Hedyotis diffusa and its adulterants from different areas were determined by the UPLC method.Conclusion The method is certified to be simple, rapid, accurate and reliable, which can be used for the determination of nucleosides in Hedyotis diffusa and its adulterants.
出处
《中国中医药信息杂志》
CAS
CSCD
2016年第7期92-94,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家自然科学基金(81274052)
山东省自然科学基金(ZR2015HL116
ZR2011HL043)
