摘要
目的:建立测定布渣叶中四个苷类成分含量的超高效液相色谱方法。方法:采用Agilent SB-C_(18)色谱柱(3.0 mm×50mm,1.8μm),以乙腈-0.05%磷酸溶液为流动相进行梯度洗脱,检测波长为320 nm,流速为0.3 ml·min^(-1),柱温为25℃,进样量:5μl。结果:布渣叶中水仙苷、异牡荆苷、山奈酚-3-O-芸香糖苷、异鼠李素-3-O-葡萄糖苷在测定范围内有良好的线性关系,其平均回收率分别为98.89%、99.03%、97.88%、97.41%,RSD分别为0.41%、0.84%、0.44%、0.80%(n=9)。结论:该方法简便快捷,且稳定性、重复性和加样回收率结果良好,可为布渣叶的质量评价和控制提供依据。
Objective： To devetop a UPLC method for determining four glycosides in microctisfolium. Methods： The UPLC deter- mination was performed on an Agilent SB - C18 （3.0 mm × 50 mm, 1.8 μm） colume with 0.05% acetonitrile - phosphate solution as the mobile phase. The detection wavelength was set at 320 nm. The flow rate was 0.3 ml· min-1. The column temperature was 25 ℃. Results： Narcissoside, isovitexin, kaempferol-3-rutinoside and isorhamnetin-3-O-glucoside all showed good linearity with the re- covery of 98.89% ,99.03%, 97.00% and 97.41%, and RSD of 0.41%, 0.84% ,0.44% and 0.80%, respectively （ n= 9 ）. Con- clusion ： The method is convenient with good stability, repeatability and sample recovery rate, which provides basis for the quality eval- uation and control of microctis folium.
出处
《中国药师》
CAS
2016年第6期1079-1081,共3页
China Pharmacist
基金
广东省中医药局建设中医药强省课题(编号:20142012)
