摘要
以木薯（Manihot esculenta Crantz）栽培种ZM–QZ1为材料,采用蛋白质组学方法,研究一天中06：00、11：00、15：00、19：00和24：00共5个时间点叶片光合作用差异蛋白的变化。结果表明：叶片在15：00时的光合速率最高,在11：00的次之,在24：00的最低;以06：00的为对照,获得其余4个时间点平均差异表达量为对照±2.0倍以上的蛋白质点42个,这些差异蛋白群的功能涉及光合作用、碳代谢、能量代谢和分子伴侣等,其中参与光合作用、碳代谢和能量代谢的蛋白质约占54.8%。与光合作用相关的关键蛋白包括核酮糖–1,5–二磷酸羧化酶/加氧酶大亚基、核酮糖–1,5–二磷酸羧化/加氧酶酶亚基结合蛋白α亚基、Rubisco活化酶和D1蛋白。这些蛋白表达水平与净光合速率在5个时间点的变化趋势一致,表明这些蛋白质的表达水平与光合作用强度密切相关。
The diurnal variation of photosynthesis-related proteins were determined using proteomics from cassava （Manihot esculenta Crantz） ZM-QZ1 leaves at five time points, including 06：00, lh00, 15：00, 19：00 and 24：00 in the whole day. The results showed that leaf net photosynthetic rate reached the highest at 15：00, followed by 11：00, and the minimum occurred at 24：00. A total of 42 differential expression proteins were found to change significantly （p〈0.05） with larger than ±2.0 times variation intensity at four time points （11：00, 15：00, 19：00 and 24：00） compared to at 06：00 time point. All differential expression proteins were identified to involve in photosynthesis, carbohydrate and energy metabolism, chaperones, etc., and 54.8% of differential expression proteins were related to photosynthesis, carbohydrate and energy metabolism. In the study, the main proteins related to photosynthesis were included in ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit, rubisco subunit binding-protein alpha subunit, ribulose-1, 5-bisphosphate carboxylase/oxygenase activase and D 1. It was indicated that the expression levels of these proteins were close related to the intensity of photosynthesis judging from the synchronous change of these protein expression levels and the net photosynthetic rate in the five time points.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2016年第3期256-261,共6页
Journal of Hunan Agricultural University(Natural Sciences)
基金
中国热带农业科学院科技攻关专题项目（1630032015005）
海南省高层次创新创业人才基金
现代农业人才支撑计划项目
关键词
木薯
光合效率
日变化
蛋白质组学
Manihot esculenta
photosynthetic efficiency
diurnal variation
proteomics
