摘要
目的:探讨新疆肉苁蓉提取液对M-KOOPG小鼠抗骨质疏松的作用机制。方法:选用M-KOOPG雌性小鼠40只,随机分成4组,即肉苁蓉提取液高、低剂量组(10,5 g·kg^(-1)·d^(-1)),福善美组(10 mg·kg^(-1)·周-1),模型组,每组各10只。连续ig给药30,60 d时每组处死M-KOOPG小鼠5只,苏木素-伊红(HE)染色观察M-KOOPG小鼠各组间骨小梁形态及数目变化;免疫组织化学法观察M-KOOPG小鼠骨组织中白细胞介素-1β(IL^(-1)β),肿瘤坏死因子-α(TNF-α)的定位及阳性表达。结果:连续ig 30,60 d,肉苁蓉提取物高、低剂量组、福善美组小鼠骨小梁形态及数目均有增加,免疫组化指标IL^(-1)β,TNF-α显示在骨小梁表面的成骨细胞、破骨细胞及骨髓基质细胞的细胞浆表达呈不同程度降低,IL^(-1)β,TNF-α阳性表达细胞数与模型组比较均有显著性差异(P<0.05)。结论:新疆肉苁蓉提取液通过降低M-KOOPG小鼠中IL^(-1)β,TNF-α的阳性表达,提示具有抗骨质疏松的作用。
Objective： To explore the anti-osteoporosis mechanisms of Xinjiang Cistanches Herba extract in M-KOOPG mice. Method： Totally 40 female M-KOOPG mice were randomly divided into 4 groups,namely the Cistanche extract high dose group and low dose group（ 10,5 g·kg^-1·d^-1）,Fosamax group（ 10 mg·kg^-1·week^- 1）,and model group,n = 10 in each group. 5 M-KOOPG mice were killed in each group respectively after continuous intragastric administration for 30 d and 60 d. Then the changes in morphology and number of bone trabecular in M-KOOPG mice were observed by hematoxylin-eosin（ HE） staining; immunohistochemistry assay was used to observe the positioning and positive expression of interleukin-1β（ IL-1β） and tumor necrosis factor-α（ TNF-α） in bone tissues of M-KOOPG mice. Result： After continuous intragastric administration for 30 d and 60 d,bone trabecular morphology and number were increased in mice of Cistanche extract high dose group,low dose group,Fosamax group, and the immunohistochemical indicators（ IL-1β, TNF-α） showed that the cytoplasm expression levels of osteoblasts,osteoclasts and bone marrow stromal cells on the bone trabecular surface were decreased in different degree. There was significant difference in cells count with IL-1β, and TNF-α positive expression as compared with the model（ P〈0. 05）. Conclusion： Xinjiang Cistanches Herba extract achieves antiosteoporosis effect in M-KOOPG mice by reducing the positive expression levels of IL-1β and TNF-α.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第10期138-142,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
新疆医学动物模型研究重点实验室开放课题(201207)
