摘要
目的探讨右美托咪定对脓毒血症大鼠肾功能及紧密连接蛋白表达的影响。方法 SD大鼠30只,随机分为右美托咪定处理组(Dex组)、脓毒血症模型组(M组)、生理盐水对照组(C组),每组10只。Dex组大鼠静脉注射脂多糖(LPS)5 mg/kg,之后泵注Dex 7μg/kg,15 min后调整剂量为5μg/kg·h,持续泵注30 min;模型组按同样方法给予LPS后泵注生理盐水;C组始终采用上述方法给予生理盐水。24 h后取血液分离血浆,多功能生化仪检测肌酐(Scr)和尿素氮(BUN)含量;ELISA检测IL-1β和TNF-α含量;Western blot检测肾组织中紧密连接蛋白ZO-1和occludin的表达变化。结果与C组相比,模型组损伤较重,可见大量炎性细胞浸润,有肾小球肾炎表现,而Dex组未见明显的炎症细胞,细胞异常较少,与模型组相比,损伤减轻。肾功能及炎性因子检测结果显示,与C组比较,各组血浆中Scr、BUN、IL-1β和TNF-α含量均显著升高(P<0.05),ZO-1和occludin蛋白表达水平均下降(P<0.05);与模型组比较,Dex组各项指标均明显降低(P<0.05),ZO-1和occludin蛋白表达水平均升高(P<0.05)。结论右美托咪定可改善脓毒血症所致的急性肾损伤的肾功能,抑制炎症反应,提升紧密连接蛋白,对肾脏有一定的保护作用。
Objective To investigate the effect of dexmedetomidine on the renal function and the expression of tight junction protein ZO-1 and occluding in the kidney of rats with septicopyemia induced by lipopolysaccharide. Methods Thirty adult male SD rats were randomly divided into 3 groups（ n = 10 each） ： control group（ group C）,LPS group（ group M）,and dexmedetomidine group（ group Dex）. In the groups Dex and LPS,the rats were infused with saline and lipopolysaccharide（ 5 mg / kg,i. v.） respectively. In the Dex group,after intravenous injection of lipopolysaccharide（ 5mg / kg）,the rats were firstly infused the loaded dose of dexmedetomidine（ 7 μg / kg） for 15 minutes,and then changed to5 μg / kg·min for 30 minutes. Blood samples were collected at 24 h later for measuring TNF-α,IL-1β,Scr and BUN. The kidney tissues were examined by histopathology. The expression of ZO-1 and occludin was detected by Western blot.Results Compared with the group C,the kidney tissue of group M was extensively damaged with tubular dilatation and inflammation,while reduced in the group Dex. Compared with the group C,the expressions of Scr,BUN,IL-1β and TNF-α were all enhanced in the groups M and Dex（ P 0. 05）,while the inflammatory factors in the group Dex were significantly lower than those of the group M（ P 0. 05）. The Results The Western blot analysis showed that the expressions of protein ZO-1 and occludin in the group Dex were significantly higher than those of the group M（ P 0. 05）.Conclusions Dexmedetomidine can improve the renal function of rats with septicopyemia,inhibit the acute renal injury and inflammation,increase the expression of protein ZO-1,and exert certain protective effect on the kidneys.
出处
《中国比较医学杂志》
CAS
北大核心
2016年第4期14-17,45,共5页
Chinese Journal of Comparative Medicine
关键词
右美托咪定
脓毒血症
肾功能
紧密连接蛋白
Dexmedetomidine
Septicopyemia
Renal function
Occludin
ZO-1
Kidney
Rat
