摘要
为了解福建地区猪伪狂犬病病毒(PRV)流行毒株的分子生物学特性与变异规律。本试验分离了2011-2013年福建省不同地区的11株PRV,并对其gD、gE基因进行遗传变异分析。结果表明,福建地区被检测的规模化猪场均存在野毒感染,病料接种PK-15细胞均能出现典型的细胞病变,分离的毒株接种家兔能出现典型的伪狂犬病症状。PRVgD、gE基因序列分析表明,自2011年福建地区PRV同源性较高且处于一个独立的分支,表明福建地区流行的PRV可能存在一定的抗原变异。
To study the genetic diversity,the molecular epidemiology and phylogenetic relationship of Pseudorabies virus（PRV）in Fujian province,the g D and g E gene in 11 PRV isolated from 2011 to 2013 were sequenced and analyzed. The resultsshowed that PRVs were found in all detected farms,and the isolated virus could cause typical cytopathogenic effect（CPE）in PK-15 cell and the rabbits injected with the isolated virus showed typical symptoms of pseudorabies,such as pruritus,palsy,opisthoto-nos and death in the end. Sequence alignments of g D and g E indicated that g D and g E genes were highly homologous,which be-long to a relatively independent sub-branch in phylogenetic analysis.We speculate that the prevalence of PRVs might have antigen-ic variation to some extent since 2011 in Fujian province.
出处
《中国兽医杂志》
CAS
北大核心
2016年第3期15-18,共4页
Chinese Journal of Veterinary Medicine
基金
福建省科技重大专项项目(2014NZ0002-3)
龙岩市科技创新平台(2013LY07)
