摘要
目的:体外评价新型根尖倒充填材料iRoot BP plus和iRoot FS的生物学性能。方法:(1)将牙根预备成长3 mm、根管直径1 mm的试样,分别于根管内填充iRoot BP plus、iRoot FS和三氧化矿物凝聚体(mineral trioxide aggregate,MTA)。将制备好的试样置于模拟体液(simulated body fluid,SBF)中,用扫描电子显微镜观察暴露的材料表面矿物沉积情况,并通过X射线能谱对其表面形成的晶体进行元素分析,测定SBF的p H值随时间的变化。(2)将iRoot BP plus、iRoot FS和MTA制备成直径8 mm、高度2 mm的圆柱,制备DMEM浸提液,通过MTT实验观察材料对MG63细胞增殖活性的影响,并通过实时荧光定量PCR和碱性磷酸酶(alkaline phosphatase,ALP)染色分析材料对ALP基因及蛋白表达的影响。结果:(1)在SBF中浸泡24 h时,iRoot BP plus、iRoot FS和MTA表面均已有矿物沉积,14 d后有大量矿物沉积,钙∶磷比值分别为1.43、1.39和1.51;(2)iRoot BP plus、iRoot FS和MTA均可使SBF的p H升高,3周时分别为8.09±0.07、7.91±0.06和8.11±0.06;(3)以体积比为1∶5和1∶10稀释的iRoot BP plus、iRoot FS和MTA浸提液对MG63细胞的增殖无明显促进或抑制作用;(4)iRoot BP plus、iRoot FS和MTA组对MG63细胞ALP基因的表达均具有促进作用,ALP染色各组未见明显差异。结论:iRoot在模拟体液中可诱导矿物质沉积,促进MG63细胞的分化和矿化,具有良好的生物活性和促成骨作用。
Objective： To study the biological characteristics of new retrograde filling materials iRoot BP plus and iRoot FS. Methods：（ 1） The roots were cut into 3 mm in length,and the root canals were prepared to 1 mm in diameter,followed by being filled with iRoot BP plus,iRoot FS,or mineral trioxide aggregate（ MTA）. The specimens were immersed in simulated body fluid（ SBF）. The ability of mineralization in vitro was detected through three studies. First,the mineralization of specimens was analyzed through scanning electron microscope observations and energy dispersive X-ray spectrometer. Then,the p H of SBF was monitored using p H meter.（ 2） The extracts were gained by immersing blocks of iRoot BP plus,iRoot FS,and MTA（ 8 mm diameter and 2 mm height） into dulbecco＇s modified eagle medium（ DMEM）. The effects of the extracts on proliferation of MG63 cells were detected through MTT assay.The gene expression level of alkaline phosphatase（ ALP） was analyzed by quantitative real-time PCR,and the expression of ALP activity was observed by ALP activity staining. Results：（ 1） The formation of minerals could be observed on the surfaces of iRoot BP plus,iRoot FS,and MTA at the end of 24 h,and there were more amounts of apatite aggregated after 14 days. The values of Ca / P ratios of apatites were1. 43,1. 39,and 1. 51,respectively.（ 2） The p H of SBF could be raised to 8. 09 ± 0. 07,7. 91 ±0. 06,and 8. 11 ± 0. 06,respectively,significantly higher than the blank.（ 3） The extracts of iRoot BP plus,iRoot FS,and MTA of dilutions of 1∶ 5 and 1∶ 10 presented no effect of proliferation of MG63 cells.（ 4） iRoot BP plus and iRoot FS could significantly up-regulated the levels of ALP messenger RNA expression,while there was no obvious difference in ALP staining among the iRoot BP plus,iRoot FS,MTA,and the blank. Conclusion： The present study shows that iRoot has displayed good mineralization capability in vitro and capability to promote differentiation and mineralization of MG63 cells,inferring that iRoot may have good bioactivity.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2016年第2期324-329,共6页
Journal of Peking University:Health Sciences
基金
国家自然科学基金项目(51372005)资助~~
关键词
根管充填材料
牙瓷料
体外研究
牙再矿化
Root canal filling materials
Dental porcelain
In vitro
Tooth remineralization
