摘要
采用高效液相色谱配备光电二极管阵列检测器法测定明日叶根、茎和叶中4-羟基德里辛（4-hydroxyderricin,4-HD）和黄色当归醇（xanthoangelol,XAG）的含量。样品分析选用Halo C（18）色谱柱（4.6 mm×150 mm,2.7μm）,流动相为0.1%甲酸（A）-甲醇（B）溶液,梯度洗脱,流速为0.3 m L/min,检测波长为370 nm,柱温为40℃。4-HD、XAG在0.945-18.900、1.048-20.960μg/m L质量浓度范围内线性关系良好,样品含量的相对标准偏差分别为3.13%、2.52%,方法的重复性好,平均回收率分别为103.70%、103.05%。经检测明日叶中4-HD的分布规律为：茎（149.16μg/g）〉根（122.42μg/g）〉叶（1.24μg/g）;XAG的分布规律为：根（144.28μg/g）〉茎（75.17μg/g）〉叶（1.21μg/g）。4-HD和XAG在根和茎中的分布显著高于其在叶中的分布。
In this study, the contents of 4-hydroxyderricin（4-HD） and xanthoangelol（XAG） in roots, stems and leaves of Angelica keiskei were determined using high performance liquid chromatography with photo-diode array（HPLC-PDA）detector. The samples were separated on a Halo C（18）-column（4.6 mm × 150 mm, 2.7 μm）, gradiently eluted with 0.1% formic acid in water（A） and methanol（B） at a flow rate of 0.3 m L/min and detected at 370 nm. The column temperature was set at 40 ℃. Good linear relationships between peak area and injection amount of 4-HD and XAG were established within the range of 0.945–18.900 and 1.048–20.960 μg/m L, respectively. The relative standard deviations（RSDs） of peak area for the two compounds were 3.13% and 2.52%, respectively, suggesting good reproducibility of the developed method. The average recoveries of the spiked compounds were 103.70%, and 103.05%, respectively. The results showed that the distribution of 4-HD in different parts of Angelica keiskei was ranked in decreasing order as follows： stems（149.16 μg/g） roots（122.42 μg/g） 〉leaves（1.24 μg/g）, while the order of XAG content was as follows： roots（144.28 μg/g）〉 stems（75.17 μg/g）〉 leaves（1.21 μg/g）. The contents of 4-HD and XAG in roots and stems of Angelica keiskei were significantly higher than in leaves.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第6期142-145,共4页
Food Science
基金
上海市科技兴农重点攻关项目(沪农科攻字(2013)第1-5号)
国家自然科学基金面上项目(21276154
31171642
31471623)
宜宾市科技厅项目(2013ZSF007)
中国博士后科学基金项目(2014M561477)
关键词
明日叶
4-羟基德里辛
黄色当归醇
高效液相色谱配备光电二极管阵列检测器
Angelica keiskei
4-hydroxyderricin（4-HD）
xanthoangelol（XAG）
high performance liquid chromatography with photo-diode array（HPLC-PDA）
