摘要
目的观察党参水提物对衰老模型小鼠肾组织micro RNA(mi RNA)表达谱的影响,探讨党参抗衰老的分子机制。方法采用皮下注射D-半乳糖溶液制造衰老模型。将100只昆明种小鼠随机分为正常组、模型组和党参低、中、高剂量组,每组20只。党参各组给予相应剂量党参水煎液灌胃干预,正常组和模型组给予等量生理盐水灌胃,连续42 d。生化分析仪测定小鼠血清尿素(BUN)和肌酐(CREA)含量;利用Affymetrixmi RNA 4.0微阵列芯片筛选D-半乳糖致小鼠衰老和党参干预衰老过程中差异表达的mi RNA,并利用生物信息学工具对芯片结果进行聚类及信号通路分析。结果与正常组比较,模型组小鼠血清BUN和CREA含量显著升高(P<0.05);与模型组比较,党参各剂量组小鼠血清BUN和CREA含量呈下降趋势,其中党参高剂量组BUN下降较显著(P<0.05);芯片聚类分析显示,模型组mi RNA表达谱与正常组和党参高剂量组明显分开,而党参高剂量组和正常组mi RNA表达谱聚在一起;另外,模型组比正常组有36个差异表达mi RNA,其靶基因参与到10个主要的生物学功能中;党参高剂量组比模型组有34个差异表达mi RNA,其靶基因的生物学功能分析与模型组比正常组的分析结果相同。结论在抗衰老过程中,党参不仅影响了mi RNA表达谱的变化,也影响了其作用的功能环境。
Objective To explore the effects of aqueous extracts of Codonopsis Radix on D-galactose induced aging model mice; To discuss the anti-aging molecular mechanism of Codonopsis Radix. Methods Subcutaneous injection of D-galactose solution was used to establish aging models. 100 Kunming mice were divided into normal control group, model group and low-, medium-, high-dose of Codonopsis Radix interventional groups randomly, 20 mice in each group. Low-, medium-, high-dose of Codonopsis Radix interventional groups were given relevant dosage for gavage, while normal control group and model group were given the same volume of NS by gavage for 42 d. After treatment for 42 days, the BUN and CREA in mouse serum were examined; the Affymetrixmi RNA 4.0 microarray was employed to identify the differentially expressed micro RNA(mi RNA) related with these processes; the bioinformatic tools were also used to further analyze the cluster of mi RNA microarrys and the pathways which the target genes of mi RNA were involved in. Results Compared with normal control group, the levels of BUN and CREA in mouse serum increased in model group(P<0.05); compared with model group, the levels of BUN and CREA in Low-, medium-, high-dose of Codonopsis Radix interventional groups decreased, in which high-dose of Codonopsis Radix could most significantly inhibit the level of BUN(P<0.05); the cluster analysis showed the mi RNA expression profilings of high dose of Codonopsis Radix interventional group and normal control group were brought together, while the profiling of model group was clearly divided with the other groups. In model group vers normal control group, 36 differentiated expressed mi RNA showed, which predicated in 10 main biological functions. In high-dose of Codonopsis Radix interventional group vers model group, 34 differentiated expressed mi RNAs in high-dose of Codonopsis Radix interventional group showed, and the analytical results of biological functions of target genes were the same as those of model group vers normal control group. Conclusion In the anti-aging process, Codonopsis Radix can not only influence the mi RNA expression profiling, but also influence its functional environment.
出处
《中国中医药信息杂志》
CAS
CSCD
2016年第5期69-72,共4页
Chinese Journal of Information on Traditional Chinese Medicine
基金
甘肃省中医方药挖掘与创新转化重点实验室开放基金项目(ZYFYZH-KJ-2015-008)
甘肃省高等学校科研项目(2013A-087)
关键词
党参
抗衰老药
MICRORNA表达谱
芯片
小鼠
Codonopsis Radix
anti-aging medicine
micro RNA expression profiling
microarray
mice
