摘要
目的:考察红托竹荪多糖(Dictyophora rubrovalvata polysaccharide,DRP)的抗衰老和降血糖作用。方法:采用水提醇沉法提取DRP。采用皮下注射D-半乳糖的方法构建衰老模型小鼠,同时DRP按低剂量(100 mg/kg·d)和高剂量(300 mg/kg·d)灌胃,35 d后测定各组小鼠血清和肝脏中超氧化物歧化酶(SOD)、谷胱甘肽-过氧化物酶(GSH-PX)活性和丙二醛(MDA)含量;给正常小鼠测血糖后皮下注射四氧嘧啶造高血糖模型小鼠,DRP按低剂量(100 mg/kg·d)和高剂量(300 mg/kg·d)灌胃,连续4周,每周测一次空腹血糖值;两组实验均设空白对照组和模型组。结果:给药300 mg/kg·d DRP可以显著增加D-半乳糖所致衰老小鼠血清和肝脏中SOD和GSH-PX活性,显著降低小鼠血清和肝脏中MDA水平;DRP能降低高血糖模型小鼠的空腹血糖,但效果不明显。结论:DRP具有延缓衰老的作用,但降血糖作用不明显。
Objective：To study anti-aging and hypoglycemic effects of Dictyophora rubrovalvata polysaccharide （DRP）in mice. Methods： DRP was extracted by using the method of water extraction and subsequent alcohol precipitation.The aging model was induced by subcutaneous injection of D-galactose, meanwhile,the mice were given different dose of DRP（ 100 mg/kg, d,300 mg/kg, d） by oral gavage.After 35 days, activity of SOD, GSH- PX and concentration of MDA in the serum and liver were determined.Hyperglycemic mice models were established by subcutaneous injection of altoxan.The mice were given different dose of DRP（100 mg/kg, d,300 mg/kg· d）by oral gavage.Fasting blood glucose was determined once a week.The two tests included blank control group and model group.Results.The activity of SOD and GSH-PX in the serum and liver increased significantly,MDA content decreased significantly.DRP could reduce the fasting blood glucose level of hyperglycemic mice, but the effect was limited.Conclusion：DRP had anti-aging effects on aging model mice but no hypoglycemic effect in mice.
出处
《食品工业科技》
CAS
CSCD
北大核心
2016年第7期343-345,共3页
Science and Technology of Food Industry
基金
贵州省科技厅
毕节市科技局
毕节学院科技联合基金项目黔科合J字LKB[2012]06号
关键词
红托竹荪
多糖
抗衰老
降血糖
小鼠
Dictyophora rubrovalvata
polysaccharide
anti- aging
hypoglycemic
mice
