摘要
目的:建立测定蒙药材刺柏叶中4种黄酮含量的一测多评法。方法:采用高效液相色谱法,以穗花杉双黄酮为内标物,建立其与槲皮苷、竹柏双黄酮A、扁柏双黄酮的相对校正因子,通过校正因子计算槲皮苷、竹柏双黄酮A、扁柏双黄酮的含量,并与外标法计算值进行比较。结果:一测多评法和外标法的计算值基本一致,相对误差均小于1.08%。结论:一测多评法用于刺柏叶中4种黄酮的含量测定是可行的。
Objective： To establish a quantitative analysis method of multi-components by single marker（QAMS）for the simultaneous determination of four flavones in Juniperus rigida leaves. Methods： By using Amentoflavone as an indicator, the relative correction factors（RCF） of Quercitrin, Podocarpusflavone A and Hinokiflavone were established. Then, the RCF was used to calculate the contents of Quercitrin, Podocarpusflavone A and Hinokiflavone. Meanwhile, the calculated value was compared with that of external standard method. Results： The calculated value of QAMS was basically the same as that of external standard method, and the relative error was less than 1.08%. Conclusion： QAMS is feasible for the simultaneous determination of 4 flavones in Juniperus rigida leaves.
出处
《中国药事》
CAS
2016年第3期297-302,共6页
Chinese Pharmaceutical Affairs
关键词
刺柏叶
相对校正因子
一测多评法
槲皮苷
竹柏双黄酮A
扁柏双黄酮
Juniperus rigida leaves
relative correction factors
quantitative analysis method of multicomponents by single marker（QAMS）
quercitrin
podocarpusflavone A
hinokiflavone
