摘要
目的：测定赶黄草袋泡茶中黄酮类成分的含量。方法：采用HPLC法,色谱柱为Kromasil C18柱（250 mm×4.0 mm,5.0μm）;流动相为甲醇-0.1%磷酸溶液（50∶50）;检测波长为360 nm;流速为1.0 m L·min-1;进样量：10μL。结果：槲皮素、山柰酚分别在0.203 6~2.036 0、0.043 2~0.432 0μg具有良好线性关系,平均回收率分别为98.43%（RSD=1.75%）和97.61%（RSD=2.17%）。结论：该方法准确、灵敏,可用于赶黄草袋泡茶中黄酮类成分槲皮素和山柰酚的含量测定。
Objective： To establish an HPLC method for the determination of quercetin and kaempferol in Ganhuangcao tea bag. Methods： The HPLC analysis was carried out on Kromasil C18（ 250 mm × 4. 6 mm,5 μm） with a mixture of methanol- 0. 1% phosphate（ 50 ∶50） as the mobile phase. The determination wavelength was set at 360 nm with the flow rate of 1. 0 m L·min- 1. Results： The quercetin and kaempferol showed good linearity in the range of 0. 2036 ~ 2. 036 0 μg and 0. 043 2 ~ 0. 432 0 μg respectively. The average recovery rate of quercetin and kaempferol were 98. 43%（ RSD = 1. 75%）and 97. 61%（ RSD = 2. 17%）,respectively. Conclusion： The method is simple and accurate. It can be used for the content determination of quercetin and kaempferol in Ganhuangcao tea bag.
出处
《中国现代中药》
CAS
2016年第2期219-221,共3页
Modern Chinese Medicine
