摘要
环境胁迫能够导致植物的DNA损伤,而DNA损伤修复基因与盐胁迫可能具有密切的相关性。根据盐穗木盐胁迫下响应的转录组测序结果,克隆获得了盐穗木具有DNA损伤修复功能的硫胺素噻唑合成酶(thiazole biosynthetic enzyme)基因,其开放阅读框1 077 bp,编码358个氨基酸,命名为HcThi1。保守结构域分析显示,保守结构域分析发现其具有SDR超家族保守结构域,合成硫胺素噻唑。系统进化树分析显示HcThi1为独立的分支,亚细胞定位于细胞质,为无信号肽的不稳定蛋白。实时荧光定量PCR分析表明,在100 mmol/L NaCl胁迫72 h后,同化枝中HcThi1的表达迅速上调并达到最大值,约为对照组的18.66倍,随着盐浓度的提高,HcThi1的表达逐渐降低。说明HcThi1基因表达受盐胁迫的诱导。研究结果有助于阐明HcThi1基因表达与植物抗盐的相关性。
Environmental stress can lead to the DNA damage of plants, and the DNA damage repair gene may have a close relationship with salt stress. According to the transcriptome of Halostachys caspica under salt stress, an cDNA fragment was isolated from Halostachys caspica. The obtained cDNA was the thiazole biosynthetic enzyme （Thil） gene ofHalostachys caspica, named as HcThil. Sequence analysis indicated that HcThil gene con- tains an open reading frame （ORF） of 1 077 bp, which encodes 358 amino acids. Conserved domain analysis showed that Hc Thil has a conserved domains of SDR protein family. Phylogenetic tree analysis indicated that Hc Thil was an independent branch, which was an stable hydrophilic proteins sub-cellularly cytoplasm. Real time quantitative PCR results showed that the expression of Hc Thil gene in branch after 100 mmol/L NaC1 salt stress for 72 h was rapidly up-regulated and reached the highest, which was 18.66-fold of the control. With the increase of salt concentration, the expression of HcThil gene gradually decreased. Base on the experimental results, the expression ofHcThil gene could be induced by salt stress. The results would help to clarify the function of HcThil gene between DNA damage-repair and salt resistance in plants.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2016年第1期206-212,共7页
Genomics and Applied Biology
基金
新疆重点实验室专项资金(2014KL001)资助
