摘要
前期我们获得了果蔗拔地拉的4份自然芽变株系,本研究旨在揭示果蔗拔地拉及其自然芽变株系在DNA水平上的分子差异。综合采用基于单引物扩增反应的三种分子标记技术(SCoT,BPS和URP)的20条单引物对果蔗拔地拉及其自然芽变株系进行分子鉴定。结果表明:(1)在随机使用的20条单引物(3条SCoT标记单引物,5条BPS标记单引物和12条URP标记单引物)中,BPS9、URP6R和URP17R这三条单引物均扩增失败,未能扩增出任何条带,剩下的17条单引物总共扩增产生了89条条带,17条单引物中平均每条单引物扩增出5.24条条带;(2)3条SCoT标记单引物和5条BPS标记单引物均未能扩增产生出明显的可重复差异条带,只有URP标记单引物URP38F能扩增产生一条由亮度强弱及有/无同时存在引起的可重复差异条带,该条带大小约625 bp,该条单引物及其所产生的约625 bp大小的条带可用于果蔗拔地拉及其自然芽变株系的分子鉴定,能有效区分果蔗拔地拉及其自然芽变株系在DNA分子水平上显示出来的微小的遗传差异。
Previously we had acquired four natural bud sports from fruit sugarcane badila. This study aimed to reveal the molecular differences between the fruit sugarcane badila and its four natural bud sports at the DNA level. Twenty single primers derived from three types of single primer amplification reaction based molecular marker techniques （SCOT, BPS and URP） were used to identify the molecular differences between the fruit sugarc- ane badila and its four natural bud sports at the DNA molecular level. The results showed that： 1） among the random used twenty single primers （three SCoT primers, five BPS primers, and twelve URP primers）, three single primers （BPS9, URP6R and URP17R） failed to amplify any bands and the remaining seventeen single primers gen- erated a total of 89 bands, with an average of 5.24 bands per primer; 2） all three single primers from SCoT marker technique and all five single primers from BPS marker technique failed to amplify obviously and reproducibly different bands, only one single primer called URP38F amplified one repeated different band which showed the different intensity of the brightness and presence and/or absence between the fruit sugarcane badila and its fournatural bud sports. The band sized approximately 625 bp. The single primer URP38F and the band sized approximately 625 bp could be used for molecular identification of fruit sugarcane badila and its four natural bud sports and effectively distinguish the tiny genetic differences between fruit sugarcane badila and its four natural bud sports at the DNA molecular level.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2016年第1期190-197,共8页
Genomics and Applied Biology
基金
国家自然科学基金项目(31501362
31401415)
广西自然科学基金项目(2014GXNSFBA118289
2014GXNSFAA118090
2014GXNSFBA118068
2015GXNSFAA139063)共同资助
关键词
甘蔗
果蔗拔地拉
芽变株系
分子鉴定
SCoT
BPS
URP
分子标记
Sugarcane, Fruit sugarcane badila, Bud sports, Molecular identification, SCOT, BPS, URP, Molecularmarkers
