摘要
为探讨P-选择素(Ps)在内毒素(LPS)致大鼠急性肺损伤(ALI)时肺组织中的表达及褪黑素(MT)对其表达的影响,将SD大鼠随机分为3组,对照(Control)组、模型(LPS)组、褪黑素干预(MT+LPS)组,采用气管内滴注脂多糖(LPS)的方法建立大鼠ALI模型,光镜下观察大鼠肺组织形态学改变,采用免疫组织化学染色技术、逆转录-聚合酶链反应(RT-PCR)和Western blot技术观察肺组织中P-选择素的表达变化.结果显示:模型组较对照组肺组织损伤严重,P-选择素的阳性表达明显增多(P<0.05);应用褪黑素干预后显著缓解上述变化(P<0.05).这说明褪黑素可能通过抑制P-选择素的表达发挥对急性肺损伤的保护作用.
To observe the expression of P-selectin（Ps） in lung tissues of acute lung injury（ALI） rat model induced by lipopolysaccharide（LPS） and examine the effect of melatonin（MT） to its expression all rats were randomly divided into three groups：Control group, LPS group and MT＋LPS group. Lung tissues were imbedded by paraffin to observe morphological changes. The auther used immunohistochemical staining technique, reverse transcription- polymerase chain reaction （ RT - PCR ） and western blot method to detect the expression of P-selectin. The results showed that compared with Control group,alveolar septum thickened significantly in LPS group,positive expression of P-selectin was very obvious（P〈0.05） ;the ad- ministration of MT mitigated above changed significantly（P〈0.05）. The results suggested that MT pos- sessed obvious protective effect on lung tissues during ALI,its protective mechanism might be related to the inhibition of the expression of P-selectin.
出处
《河北大学学报（自然科学版）》
CAS
北大核心
2016年第1期78-83,共6页
Journal of Hebei University(Natural Science Edition)
基金
河北省科技厅资助项目(08276101D-88)
