摘要
目的观察辣椒素对IL-1β诱导的人肺腺癌NCI-H460细胞PGE_2含量的影响,并且进一步观察其对COX-2和mPGES-1的影响,探讨其抗非小细胞肺癌(NSCLC)的可能机制。方法体外培养NCI-H460细胞,采用MTT比色分析法,观察辣椒素对NCI-H460细胞增殖抑制作用,计算IC50;采用IL-1β刺激的方法构建炎症模型,ELISA法检测辣椒素对NCI-H460细胞PGE_2含量和COX-2活性的影响;Western blot法检测辣椒素对NCI-H460细胞COX-2、mPGES-1蛋白表达的影响;Real-time PCR法检测辣椒素对NCI-H460细胞COX-2mRNA和mPGES-1mRNA表达的影响。结果 MTT比色分析结果表明,辣椒素对NCI-H460细胞增殖具有明显抑制作用,与对照组比较,差异具有统计学意义(P<0.05或P<0.01)。辣椒素明显降低NCI-H460细胞COX-2活性和PGE_2浓度,而且明显降低NCIH460细胞COX-2、mPGES-1蛋白及其mRNA的表达,且呈剂量依赖性,与模型组比较,差异具有统计学意义(P<0.05)。结论辣椒素通过降低NCI-H460细胞COX-2和mPGES-1 mRNA表达从而抑制PGE_2释放,可能是其抗NSCLC的机制之一。
Objective To observe the effects of capsaicin on PGEz concentration of IL-1β-induced human large cell carcinoma NCI-H460 cells, and further observe its effect on COX-2 and mPGES-1 so as to explore the possible mechanisms against non-small cell lung cancer. Methods NCI-H460 cells were cultured in vitro; the effect of capsaicin in inhibiting NCI-H460 cells proliferation was observed. The 50% inhibitory concentration （IC50） was measured by MTT assay. IL-1β stimulation method was used to construct inflammation model, and the effects of capsaicin on COX-2 activity and PGE2 concentration in NCI-H460 cells were measured by ELISA. The effects of capsaicin on COX-2 and mPGES-1 protein level in NCI-H460 cells were analyzed by Western blot; the effects of capsaicin on COX-2 mRNA and mPGES-1 mRNA expressions in NCI-H460 cells were analyzed by Real-time PCR. Results MTT assay results showed that the growth of NCI-H460 cells treated with capsaicin was significantly inhibited compared with the control group （P〈0.05 or P〈 0.01）. Capsaicin could significantly decrease COX-2 activity and PGE2 concentration in NCI-H460 cells, and significantly decrease COX-2, mPGES-1 protein levels as well as COX-2, mPGES-1 mRNA expressions in NCI-H460 cells in a dose-dependent manner compared with the control group （P 〈0.05）. Conclusion Capsaicin inhibits the release of PGE2 by downregulating COX-2 and mPGES-I mRNA expressions in NCI-H460 cells, which may be one mechanism of its effect against non-small cell lung cancer.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2016年第2期283-287,306,共6页
Journal of Xi’an Jiaotong University（Medical Sciences）
