摘要
目的探讨益气活血方防治放射性肺损伤的分子生物学机制和相关敏感因子。方法将108只雌性SD大鼠采用随机数字表法分为中药组（A组）、单纯照射组（B组）及健康对照组（C组）,每组36只。应用6MV-X直线加速器对前2组大鼠右肺进行照射（5 Gy/次,2次/周,累积剂量为30 Gy）,A组于受照射当天开始用益气活血方1 ml灌胃、2/d,B组及C组用蒸馏水1 ml灌胃、2/d,停止时间为照射结束后2周。各组于照射开始第2、4、8、12、16、20周末随机抽取6只大鼠处死,取其肺组织,用反转录-聚合酶链反应（RT-PCR）及免疫组化方法检测大鼠肺组织内皮型一氧化氮合酶（NOS3）、亚甲基四氢叶酸还原酶（MTHFR）mRNA及蛋白表达。结果 A组与B组NOS3、MTHFR的mRNA及蛋白表达于照射后第2周开始增高,第8周达高峰,第12周开始下降,16周后降低明显,与C组相比,差异均有统计学意义（P〈0.01）;A组与B组相比,NOS3、MTHFR mRNA及蛋白表达趋势类似,A组表达水平在12周之前均高于B组,差异有统计学意义（P〈0.05,P〈0.01）。结论 NOS3、MTHFR可能为放射性肺损伤的敏感因子。益气活血方可通过上调NOS3、MTHFR的表达起到减轻放射性肺损伤的作用。临床放射性肺损伤治疗时间应延长2~3个月,各时间段治疗重点应有所侧重。
Objective To investigate the molecular biological mechanisms and related sensitive factors of supplementing Qi and activating blood circulation of traditional Chinese drug in prevention and treatment of radiation induced lung injury （RILI）. Methods A total of 108 SD male rats were divided into traditional Chinese drugs group （ group A, n = 36） , only irradiation group （group B, n = 36） and healthy control group （group C, n = 36） with random digits table. Group A and B were irradiated by 6MV-X linear accelerator on right lung with 30 Gy accumulated dose （5 Gy/time, 2 times/week）. On the 1^st d of radiation, group A was gavaged with 1 ml supplementing Qi and activating blood circulation prescription （2/d） , and group B and C were gavaged with 1 ml distilled water （2/d） , and the gavage was stopped 2 weeks after the irradiation. Six rats were randomly selected from each group at the end of the 2^nd , 4^th , 8^th , 12^th , 16^th and 20＇h week after the irradiation beginning, and were sacrificed to obtain the lung tissues, and then the messenger ribonucleic acid （mRNA） and protein expressions of endothelial nitric-oxide synthase （ NOS3 ） and methylenetetrahydrofolate reduetase （MTHFR） in lung tissues were measured using reverse transcription-polymerase chain reaction （RT-PCR） and immunohistochemistry methods. Results Compared with those in group C, mRNA and protein expressions of NOS3 and MTHFR were increased in the 2^nd week after the irradiation beginning, and the peak was found in the 8^th week after the irradiation beginning, and then the expressions were decreased in the 12^th week after the irradiation beginning, and the obvious decline was found in the 16^th week after the irradiation beginning, and the differences were statistically significant （P 〈0.01）. Compared with those in group B, in group A, mRNA and protein expressions of NOS3 and MTHFR had similar trend, but the expressions were higher until 12^tb week after the irradiation beginning, and the differences were statistically significant （P 〈 0. 05, P 〈 0.01 ）. Conclusion NOS3 and MTHFR may be RILl sensitive factors. The supplementing Qi and activating blood circulation prescription may lighten RILl by increasing the NOS3 and MTHFR expression. The RILl clinical therapy should be prolonged for two or three months. The therapeutic emphasis should be different at different stages.
出处
《解放军医药杂志》
CAS
2016年第2期38-41,51,共5页
Medical & Pharmaceutical Journal of Chinese People’s Liberation Army
基金
全军"十二五"中医药重点项目(10ZYZ105)
关键词
放射性肺损伤
益气活血方
内皮型一氧化氮合酶
亚甲基四氢叶酸还原酶
大鼠
Sprague-Dawley
Radiation induced lung injury
Supplementing Qi and activating blood circulation prescription
Endothelial nitric-oxide synthase
Methylenetetrahydrofolate reductase
Rats, Sprague-Dawley
