摘要
目的研究S期激酶相关蛋白2(S-phase kinase associated protein 2,Skp2)在前列腺癌患者中的表达及其与前列腺癌术后复发的关系。方法选择2010年6月-2012年6月接受手术的前列腺癌86例作为观察组,同时选取良性前列腺增生25例作为对照组,检测两组前列腺组织中Skp2的mRNA水平和Skp2蛋白的表达,根据Skp2表达情况将观察组分为阳性组和阴性组,分析Skp2表达与临床病理特征及术后复发的关系。结果与对照组相比,观察组前列腺癌组织中Skp2mRNA和Skp2蛋白的表达均显著升高(P<0.05)。观察组Skp2阴性35例的1、2、3年复发率为8.57%、25.71%、31.43%,显著低于Skp2阳性51例的27.45%、39.22%、49.02%(P<0.05);Skp2阴性患者1、2、3年生存率分别为97.14%、91.43%、82.86%,显著高于Skp2阳性患者的90.19%、84.31%、70.59%(P<0.05)。结论前列腺癌组织中Skp2 mRNA和Skp2蛋白的高水平表达与前列腺癌组织分型、临床分期、肿瘤浸润程度以及淋巴结转移有一定关系,可能是前列腺癌发展的重要机制之一。
Objective To study the expression of Skp2 in prostate cancer and its relationship with the recurrence of prostate cancer. Methods 86 cases of patients diagnosed with prostate cancer and received operation in our hospital from June 2010 to June2012 were randomly selected as observation group. At the same time,25 cases patients with benign prostatic hyperplasia were selected as control group. Skp2 mRNA level in the two groups were determined by real-time quantitative PCR. Skp2 protein level was analyzed by immunohistochemistry. According to Skp2 level,86 cases of patients were divided into Skp2 positive group and Skp2 negative group.The relationship between Skp2 protein level and clinical pathological features and postoperative recurrence in Skp2 positive group and Skp2 negative group were analyzed. Results Compared with the control group,Skp2 mRNA and protein levels in the prostate cancer tissues of the observation group increased significantly（ P0.05）. 1,2 and 3 year cumulative recurrence rate in the Skp2 negative patients were 8.57%,25.71 and 31.43%,while 27.45%,39.22% and 49.02% in Skp2 positive patients. The cumulative recurrence rate of Skp2 protein-negative patients was significantly lower than that of Skp2 positive patients（ P0.05）. 1,2 and 3 year cumulative survival rate in the Skp2 protein-negative patients were 97.14%,91.43% and 82.86%,while 90.19%,84.31% and 70.59% in Skp2 positive patients. The cumulative survival rate of Skp2 protein-negative patients was significantly higher than that of Skp2 positive patients（ P0.05）. Conclusion The high expression of Skp2 mRNA and protein in prostate cancer tissues are closely related to types,clinical stage,tumor invasion and lymph node metastasis of prostate cancer,which may be one of the important mechanism of prostate cancer development.
出处
《东南国防医药》
2016年第1期47-50,共4页
Military Medical Journal of Southeast China
