摘要
目的采用SPE-HPLC联用技术测定风湿骨痛片麻黄中盐酸麻黄碱和盐酸伪麻黄碱的含量。方法采用Oasis MCX-SPE阳离子固相萃取小柱对样品进行前处理,Welch XDB-C18(250mm×4.6mm,5μm)为色谱柱,以乙腈-0.1mL·L^(-1)磷酸(用三乙胺调pH值至3.0)(5∶95)为流动相等度洗脱,流速1.0mL·min^(-1),检测波长207nm,柱温30℃。结果该实验条件下,盐酸麻黄碱和盐酸伪麻黄碱分离度好。盐酸麻黄碱进样量在22.99~1 150ng、盐酸伪麻黄碱进样量在14.60~730.2ng范围内线性关系良好,盐酸麻黄碱平均回收率为100.8%(RSD=2.8%),盐酸伪麻黄碱平均回收率为98.6%(RSD=2.4%)。结论该方法简便、专属性好,能有效消除复方中其他干扰,可用于风湿骨痛片中麻黄的质量控制。
Objective To establish a method for the content determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Fengshigutong Tablets by SPE-HPLC.Methods Samples were pretreated by Oasis MCX-SPE cation solid phase extraction column and analyzed by HPLC consisting of a Welch XDB-C18（250mm×4.6mm,5μm）column and a mixture of acetonitrile-0.1mL·L^-1 H3PO4（adjusting the pH to 3.0 with triethylamine）（5∶95）solution as the mobile phase,flow rate was1.0mL·min^-1 and wavelength was 207 nm.The column temperature was 30 ℃.Results The method showed good linearity in the range of 22.99～1 150 ng and 14.60-730.2ng.The average recovery of ephedrine hydrochloride was 100.8%（RSD=2.8%）,pseudoephedrine hydrochloride 98.6%（RSD=2.4%）.Conclusion This method can eliminate the negative interference,with good specificity,and can be used for the quality control of Fengshigutong Tablets.
出处
《西北药学杂志》
CAS
2016年第1期45-48,共4页
Northwest Pharmaceutical Journal
关键词
风湿骨痛片
盐酸麻黄碱
盐酸伪麻黄碱
SPE-HPLC
含量测定
Fengshigutong Tablets
ephedrine hydrochloride
pseudoephedrine hydrochloride
SPE-HPLC
content determination
