摘要
目的：通过对高胆红素血症新生儿进行新生儿溶血症的血清学检测，评价血清学试验在新生儿溶血病诊断中的价值，分析其与血清胆红素浓度的相关性。方法用微柱凝胶法检测卡进行溶血三项试验，同时进行血清胆红素的检测，并对结果进行统计学分析。结果265例母婴血型不合病例中，抗体释放试验阳性率为54.3%，游离抗体试验阳性率为49.1%，直接抗人球蛋白试验阳性率为17.0%。高胆红素血症占47.2%（125/265），血清学试验阳性组高胆红素血症的发生率显著高于试验阴性组（χ2=32.36，P 〈0.01）和对照组（χ2=54.58，P 〈0.01）。直接抗人球蛋白试验、游离抗体试验以及抗体释放试验阳性率与血清胆红素浓度未见明显相关性（P 〉0.05），游离抗体试验与抗体释放试验有很强的相关性（r =0.897，P 〈0.01）。结论溶血三项血清学试验中直接抗人球蛋白试验诊断的特异度最高，抗体释放试验诊断的敏感度最高。血清学试验阳性率与血清胆红素浓度无明显相关性，游离抗体试验与抗体释放试验有很强的相关性。
Objective To evaluate the diagnostic value of direct antiglobulin test,free antibodies test and antibody releasing test in hemolytic disease of newborn and analyze the correlation of the incidence rate of hyperbilirubinemia and the density of ser-um bilirubin with serological test. Methods Microcolumn gel test was used to detect the three hemolysis test,and the density of serum bilirubin at the same time. Results Among the 265 maternal-fetal blood,the positive rate of antibody releasing test was 54. 3% ,the free antibodies test was 49. 1% and the direct antiglobulin test was 17. 0% . The rate of hyperbilirubinemia was 47. 2% ,the incidence rate of hyperbilirubinemia in positive serological test group was higher than that of negative serological test group（χ2 = 32. 36,P 〈 0. 01）and that of control group（χ2 = 54. 58,P 〈 0. 01）. There were no significant correlation of direct antiglobulin test,free antibodies test and antibody releasing test with the density of serum bilirubin. There was significant correla-tion of free antibodies test with antibody releasing test（r = 0. 897,P 〈 0. 01）. Conclusion The highest sensitivity is antibody re-leasing test and the highest specificity is direct antiglobulin test among three hemolysis tests. The correlation is not significant of he-molysis test with the density of serum bilirubin. There is significant correlation of free antibodies test with antibody releasing test.
出处
《临床医学》
CAS
2015年第12期41-43,共3页
Clinical Medicine
关键词
高胆红素血症
新生儿溶血症
血清学试验
Hyperbilirubinemia
Hemolytic disease of newborn
Serological test
