摘要
目的研究间接免疫荧光法(IIF)检测抗核抗体(ANA)与印迹法(LIA)检测ANA谱(ANAS)的诊断结果,并分析二者的一致性及相关性。方法选取2013年2月至2014年11月在该院风湿科疑为自身免疫性疾病(AID)的住院及门诊患者为研究对象,采用IIF与LIA分别检测ANA与抗核抗体谱ANAS,并对结果进行统计分析。结果检出ANA阳性共127例,ANAS阳性84例,最终确诊AID 96例,各种特异性抗体均有检出,优势性检验发现总体阳性率差异有统计学意义。结论使用IIF检测ANA的阳性率高于LIA,但对于疾病的诊断价值,则LIA检测ANAS优于IIF检测ANA,临床上二者不可互相代替,应联合检测,防止漏检。
Objective To study the diagnostic results of the indirect immunofluorescence（IIF）and blot method for detecting ANA spectrum,and to analyze their consistency and correlation.Methods The inpatient and outpatient with suspected autoimmune diseases in the rheumatism department of our hospital from February 2013 to November 2014 were selected as the research subjects.The IIF method and blot method were adopted to detect antinuclear antibody（ANA）and ANA spectrum（ANAS）.Then the detection results were analyzed.Results 127 cases of ANA positive were detected out,84 cases of ANAS positive and 96 cases were finally diagnosed as autoimmune diseases（AID）,all kinds of specific antibody were detected,the dominance detection found that there was difference among the total positive rate（P〈0.05）,which showing the significance.Conclusion Using IIF for detecting ANA has higher positive rate than the blot method,but for the diagnostic value of disease,the blot method for detecting ANAS is superior to IIF for detecting ANA.But both two method cannot be replaced each other in clinic,their combined detection should be conduced for preventing missing detection.
出处
《国际检验医学杂志》
CAS
2016年第1期53-54,57,共3页
International Journal of Laboratory Medicine
关键词
间接免疫荧光法
印迹法
抗核抗体
相关性
自身免疫性疾病
indirect immunofluorescence method
blot method
antinuclear antibody
correlation
autoimmune diseases
