摘要
以耐盐植物乳杆菌FS5-5为研究对象,采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳技术构建了菌株在Na Cl质量浓度分别为0、3、6、9 g/100 m L的培养基中生长至对数期中期的全蛋白表达图谱。通过比较分析,选取了6个差异蛋白质条带,并采用液相色谱-质谱/质谱联用对差异蛋白质条带进行质谱分析。结果表明:1号样品条带鉴定得到6种蛋白;2号样品条带鉴定得到11种蛋白;3号样品条带鉴定得到9种蛋白;4号样品条带鉴定得到4种蛋白;5号样品条带鉴定得到15种蛋白;6号样品条带鉴定得到15种蛋白。去除相同的蛋白,共有45种蛋白得到鉴定。这些蛋白大致可以分为4类:与蛋白质合成有关的蛋白25种;与代谢相关的蛋白10种;与核苷酸合成有关的蛋白8种;未知功能蛋白2种。可能由于这些蛋白的表达发生变化,才导致菌体中蛋白质合成、能量代谢、DNA复制能够正常进行,最终使植物乳杆菌FS5-5能够更好地在盐环境下生存下去。
In this study, the total protein profiles of Lactobacillus plantarum FS5-5 with salt tolerance grown in media with Na Cl concentrations of 0, 3, 6, and 9 g/100 m L to the middle logarithmic phase were established and compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis（SDS-PAGE）, and six different protein bands were selected for analysis by liquid chromatography-tandem mass spectrometry（AB Sciex 4000 QTRAP LC/MS/MS）. Results showed that 6, 11, 9, 4, 15 and 15 proteins were contained in bands 1, 2, 3, 4, 5 and 6, respectively. Totally 45 proteins were identified. These proteins could be divided into 4 groups including 25 proteins involved in protein synthesis, 10 proteins associated with metabolism, 8 proteins associated with nucleotide synthesis, and 2 proteins with unknown functions. The differential expression of these proteins could lead to bacterial protein synthesis, energy metabolism and DNA replication, and eventually enable Lactobacillus plantarum FS5-5 to better survive in salt stress environment.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2015年第23期155-161,共7页
Food Science
基金
国家自然科学基金面上项目(31000805
31471713)
辽宁省农业领域青年科技创新人才培养资助计划项目(2014048)
辽宁省高等学校优秀人才支持计划项目(LR2015059)
江苏省博士后科研资助计划项目(1402071C)
沈阳农业大学“天柱山英才支持计划”项目
关键词
植物乳杆菌FS5-5
盐胁迫
十二烷基硫酸钠-聚丙烯酰胺凝胶电泳
差异蛋白
Lactobacillus plantarum FS5-5
salt stress
sodium dodecyl sulfate-polyacrylamide gel electrophoresis（SDS-PAGE）
different proteins
