摘要
在生物催化中,很多的复杂反应都需要2个或者多个酶的级联反应催化得到,近几年多酶生物催化开始逐渐取代单酶和细胞发酵,成为酶工程发展的一个热点研究方向。本文对多酶催化剂的制备形式和原理及超分子酶在级联催化中的应用进行了综述。其中超分子酶的制备技术主要包括非定向的固定化技术和定向的基因融合技术、纤维小体支架技术和DNA支架技术等。
In bio-catalytic reactions,many cascade reactions need two or more enzymes to catalyze the reaction. In recent years,multi-enzyme reactions have begun to replace single enzyme and cell catalyzed reactions,and have becomes an important research direction in the development of enzyme engineering. In this paper,the methods and theories of preparation of multi-enzyme complexes and their applications in cascade reactions are reviewed. Methods of preparation of supramolecular enzymes discussed include undirected immobilization and directed gene fusion,cellulosome scaffolds and DNA scaffolds.
出处
《北京化工大学学报（自然科学版）》
CAS
CSCD
北大核心
2014年第5期1-8,共8页
Journal of Beijing University of Chemical Technology(Natural Science Edition)
基金
国家"973"计划(2013CB733600)
关键词
酶
级联催化
超分子酶
enzyme
cascade reaction
supramolecular enzyme
