摘要
目的探讨养阴活胃合剂对慢性萎缩性胃炎模型大鼠B淋巴细胞瘤-2基因(B-cell lymphoma-2,Bcl-2)和三叶因子1(trefoil factor 1,TFF1)基因蛋白表达的影响及机制。方法将实验大鼠随机分为空白对照组、模型组、中药低、中、高剂量组及阳性药物对照组,每组16只。除空白对照组常规饲养、自由进食水外,均采用2 g水杨酸钠加入100 m L的30%乙醇溶液中给大鼠灌胃,每天1次,每次3 m L,配合隔日喂食不禁水法造成慢性萎缩性胃炎模型,模型复制成功后分别运用中药低、中、高剂量及维酶素治疗,采用免疫组化法检测大鼠胃黏膜Bcl-2及TFF1表达阳性细胞数,光镜观察胃黏膜病理组织学变化。结果采用单因素方差分析法,组间比较采用方差分析中的LSD法。与空白对照组(18.08%)比较,各组Bcl-2蛋白阳性表达率均明显升高(P<0.05);与模型组(43.41%)比较,应用养阴活胃合剂治疗后,中药高、中剂量组Bcl-2蛋白阳性表达率均明显降低(P<0.05)。与空白对照组(95.66%)比较,各组TFF1蛋白阳性表达率均明显降低(P<0.05),除中药高剂量组TFF1蛋白阳性表达率接近于空白对照组外(P>0.05);与模型组(84%)比较,中药高、中剂量组TFF1蛋白阳性表达率均明显升高(P<0.05)。结论养阴活胃合剂对CAG大鼠模型有良好的治疗作用,能够改善慢性萎缩性胃炎大鼠胃黏膜病理形态,提示养阴活胃合剂可能通过下调Bcl-2的表达,上调TFF1表达起到治疗效果。
Objective To explore the influence and mechanism of Yangyin Huowei mixture in Bcell lymphoma 2 gene（ B-cell lymphoma-2,Bcl-2） and trefoil factor-1（ TFF-1） gene expression of chronic atrophic gastritis rats model. Methods The experimental rats were randomly divided into control group,model group,low dose,middle dose,high dose group and positive control group,16 rats in each group.Control group were treated with conventional farming,free access to water and food. Other groups were gavage administration solution which was sodium salicylate 2g adding in 100 ml of 30% ethanol,1 times a day,each time 3ml. And the rats were feed with alternate days to induced chronic atrophy gastritis model.When the model was successful,the rats were gavage administration with chinese medicine and riboflavin.Immunohisto chemical method was used to detect positive cells of Bcl-2 and TFF-1,and light microscope was used to observe the changes of gastric mucosa. Results The results were compared with single factor analysis of variance,and the comparison among groups was performed with LSD method. Compared with the control group（ 18. 08%）,the positive expression rate of bcl-2 protein were significantly increased（P〈0. 05）; and compared with the model group（ 43. 41%）,positive expression rate of bcl-2 protein were significantly decreased in Yangyin Huowei mixture high and middle dose group（P〈0. 05）. Compared with the control group（ 95. 66%）,the positive expression rate of TFF-1 protein was significantly decreased（P〈0. 05） in other groups. The positive expression rate of TFF-1 protein in the high dose group was close to control group（P〈0. 05）; the positive expression rate of TFF-1 protein in Yangyin Huowei mixture high and middle dose group was significantly higher than that in model group（ 84%）. Conclusion Yangyin Huowei mixture in treatment of CAG rat model has good effect,and it can improve the pathological morphology of chronic atrophic gastritis in rat. all above show that Yangyin Huowei mixture can down-regulation of bcl-2 expression and up regulation of TFF-1 expression to have a good therapeutic effect.
出处
《环球中医药》
CAS
2015年第12期1437-1440,共4页
Global Traditional Chinese Medicine
基金
国家自然科学基金(81160431)
