摘要
目的比较两种RNA提取方法对丙型肝炎病毒(hepatitis C virus,HCV)RNA检测结果的影响,为临床实验室质量控制和结果评价提供依据。方法以定值质控品为标准物质,采用硫氰酸胍法和蛋白酶K法两种RNA提取方法对我院2014年11月确诊的33例HCV感染者进行HCV RNA定量分析。结果两种检测方法检测定值质控品的批内不精密度分别为3.7%和2.2%、批间不精密度分别为5.0%和4.3%。硫氰酸胍法提取定值质控品RNA进行定量PCR检测结果 lg值为4.15±0.21低于蛋白酶K法的4.80±0.21,差异有统计学意义(t=10.88,P<0.001);两种方法对本组HCV感染者检测结果 lg值分别为4.86±1.14和5.29±1.22,差异有统计学意义(t=12.10,P<0.001),且二者呈显著线性相关(r=0.987,P<0.001)。结论与硫氰酸胍法比较,蛋白酶K法是一种效率更高,检测数据更准确的RNA提取方法。
Objective To compare the effects of two ribonucleic acid（ RNA） extraction assays on RNA quantitative analysis for Hepatitis C virus（ HCV） in order to provide evidence for quality control and result evaluation. Methods The quantitative material of quality control was used as standard substance,and RNA quantitative analysis was performed using two extraction assays with Guanidinium Thiocyanate and protease K for 33 HCV infected patients admitted in November 2014. Results Intra-batch imprecision values of the two RNA extraction assays were 3. 7% and 2. 2%,and inter-batch imprecision values were 5. 0% and 4. 3% respectively. For the constant quality control,the lg value 4. 15 ±0. 21 using the Guanidinium Thiocyanate assay was lower than 4. 80 ± 0. 21 using the protease K assay,and the difference was statistically significant（ t = 10. 88,P〈0. 001）. For the serum of HCV infected patients,the lg value 4. 86 ± 1. 14 using the Guanidinium Thiocyanate assay and 5. 29 ± 1. 22 using the protease K assay,and the difference was statistically significant（ t = 12. 10,P〈0. 001）. The results showed that significant linear correlations existed in HCV quantitative detection by different test assays（ r = 0. 987,P〈0. 001）. Conclusion The Guanidinium Thiocyanate assay is a more efficient and accurate method for RNA extraction compared with the protease K assay.
出处
《解放军医药杂志》
CAS
2015年第12期71-74,共4页
Medical & Pharmaceutical Journal of Chinese People’s Liberation Army
