摘要
目的探讨金银花-荆芥穗不同配比乙醇提取物对流感病毒感染的MDCK细胞的作用及其有效成分含量的变化，筛选出最佳剂量配比关系。方法以利巴韦林为阳性对照药物，通过MTT比色法检测经不同配比金银花-荆芥穗溶液混合后对流感病毒感染的MDCK细胞存活率，研究药对的抗流感病毒作用，并采用反相高效液相色谱法对提取物中绿原酸、木犀草苷和木犀草素的含量进行了测定。结果金银花．荆芥穗不同配比（1：0、0：1、1：1、2：1、3：1、4：1）提取物对流感病毒的治疗指数分别为34．83、32．92、23．26、87．06、37．08、24．71，而利巴韦林对照的治疗指数为35．90。当药物浓度均为2．0mg／mL时．金银花．荆芥穗2：1组的病毒抑制率为（94．54±2．25）％，显著优于利巴韦林的病毒抑制率【（64．77±7．89）％1（f=7．25，P〈0．01）。结论金银花-荆芥穗不同配伍比例均有抗流感病毒作用，当金银花-荆芥穗以2：1配伍时，抑制流感病毒的能力最强。
Objective To discuss the ethanol extracts from honeysuckle-schizonepeta spike （HSS） against influenza virus in MDCK cell and content changes of the active ingredients in HSS with different compatibilitity ratios were studied in order to screen out the best compatibility ratio. Methods The viability of influenza virus infected MDCK cell mixed with HSS was detected by MTT assay using ribavirin as a positive control drug, and the antiviral effect was evaluated. Meanwhile, the contents of chlorogenic acid, luteoloside and luteolin in HSS were determined by reversed-phase high performance liquid chromatography. Results Therapeutic index （TI） of HSS with different compatibilities （ 1 ： 0, 0 ： 1, 1 ： 1, 2 ： 1, 3 ： 1 and 4 ： 1 ） against influenza virus were 34.83, 32.92, 23.26, 87.06, 37.08 and 24.71 respectively, while the TI of ribavirin was 35.90. The virus inhibition rate of HSS with the 2 ： 1 group was （94.54± 2.25）%, which was significantly better than ribavirin group [（64.77±7.89）%] when the drug concentrations were both 2.0 mg/mL （t=7.25, P〈0.01 ）. Conclusions HSS with different compatibilities all exert anti-influenza virus effect, and HSS with the compatibility ratio of 2 ： 1 inhibits influenza virus replication best.
出处
《国际流行病学传染病学杂志》
CAS
2015年第6期371-375,共5页
International Journal of Epidemiology and Infectious Disease
基金
国家自然科学基金（81202977）
浙江省自然科学基金（LQ15H280007）
浙江省中医药科技计划项目（2016ZA042,2013ZA026）
关键词
药对
金银花-荆芥穗
配伍比例
流感病毒
抗病毒作用
Chinese medicine pair
Honeysuckle-schizonepeta spike
Compatibility ratio
Influenza virus
Antiviral effects
