摘要
采用组织分离法和土壤稀释涂板法,对元阳哈尼梯田2个地方品种‘月亮谷'和‘红脚老粳'的根部内生细菌及根际土壤细菌进行了分离,石开究元阳梯田传统水稻品种特殊的内生菌组成.结果表明:试验共得到399个菌株.经形态特征及生理生化鉴定,月亮谷根部和其根际土壤分别分离到8和5个属,其中5个属是共有的;红脚老粳的根部和其根际土壤中分别分离到10和7个属,其中6个属是共有的.经分子生物学鉴定,月亮谷根部分离到11个种和5个属,根际土壤分离到8个种和4个属,其中5个种和4个属是共有的;红脚老粳根部分离到9个种和5个属,根际土壤分离到10个种和3个属,其中4个种和2个属是共有的.通过分子生物学鉴定,大部分菌株都可以鉴定到种,而通过形态及生理生化特性只能初步鉴定到属.但两种方法在属层次上的鉴定结果基本一致.元阳地方水稻根部内生细菌及根际土壤细菌具有一定的种属同源性与特异性.
To reveal root endophytic and rhizosphere bacteria constitution of rice landraces in Yuanyang Terrace,isolation was carried out by tissue isolation method and soil dilution plate method for two landraces of Yuelianggu and Hongjiaolaojing.A total of 399 bacterial strains isolated were identified by morphological characteristics,physiological and biochemical identification.The results showed that there were 8 genera isolated from the root of Yuelianggu and 5 genera from its rhizosphere soil,and 5 genera were same.For Hongjiaolaojing,there were 10 genera isolated from its root and 7 genera from its rhizosphere soil,and 6 genera were same.By molecular biology identification,a total of 11 species and 5 genera were isolated from the root of Yuelianggu,8 species and4 genera from its rhizosphere soil,and 5 species and 4 genera were same.As for Hongjiaolaojing,there were 9 species and 5 genera isolated from its root,and 10 species and 3 genera from its rhizosphere soil,and 4 species and 2 genera were same.The results of physiological and biochemical characteristics identification method and molecular identification method were basically same at the genus level,while most of the strains could be identified to species by molecular identification.There were certain species homology and specificity in the root endophytic and rhizosphere bacteria of Yuanyang rice landraces.
出处
《应用生态学报》
CAS
CSCD
北大核心
2015年第12期3737-3745,共9页
Chinese Journal of Applied Ecology
基金
国家基础研究发展计划重点项目(2011CB100400)资助
