摘要
目的探讨消银2号对豚鼠耳部银屑病样皮损组织病理学及增殖细胞核抗原(PCNA)的影响。方法健康豚鼠48只,随机分为空白组、模型组、当归饮子组(9.88 g·kg^(-1))、消银2号高、中、低剂量组(26.7g·kg^(-1)、13.4g·kg^(-1)、6.68g·kg^(-1))。采用5%心得安诱导法建立豚鼠银屑病样模型,连续造模3周后各组开始按剂量灌胃,空白组、模型组给予生理盐水,连续2周。检测豚鼠耳组织Baker评分、皮损PCNA的含量。结果消银号高、中、低剂量组及当归饮子组组织病理学Baker评分[分别为(3.200±0.570)、(2.600±0.418)、(2.750±0.524)、(3.000±0.447)]均低于模型组(4.643±0.476)(P<0.05);消银2号高、中、低剂量组皮损中PCNA含量[(14.645±0.751)、(14.153±0.899)、(14.918±0.652)]均低于模型组(16.889±0.883)(P<0.05)。结论消银2号可通过改善组织学病理评分、抑制PCNA的表达发挥治疗银屑病的作用。
Objective To study the Xiaoyin No.2＇ effect of content on the ear skin histopathological and proliferating cell nucleus antigen （PCNA） in psoriatic lesions of guinea pig. Methods 48 healthy guinea pigs were randomly divided into blank group, model group, angelica Yinzi（9. 88 g.kg-1）, Xiaoyin No.2 high, medium and low dose group（26. 7 g.kg-1, 13. 4 g.kg-1, 6. 68 g.kg-1）. 5% pmpranolol induction method was adopted to establish the guinea pig model psoriasis samples, for building 3 weeks after each group started by ig, the blank group and model group were given saline ig, for 2 weeks. Detection of guinea pig ear tissue Baker score, lesion of PCNA content. Results Xiaoyin No.2 high, medium and low dose group and angelica Yinzi histopathological grading Baker[respectively（3. 200±0.570）, （2. 600±0.418）, （2. 750±0.524）, （3. 000±0.447）] were lower than that of model group[（4. 643±0.476）]（P〈0.05） ;Xiaoyin No.2＇ high, medium and low dose group of PCNA content[（ 14. 645±0.751 ）, （14. 153±0.899）, （14. 918±0.652）] were lower than that of model group （16. 889±0.883）（P〈0.05）. Conclusion Xiaoyin No.2 can play a role for the treatment of psoriasis by improving the histological pathology score and inhibit the expression of PCNA.
出处
《云南中医学院学报》
2015年第6期13-16,共4页
Journal of Yunnan University of Traditional Chinese Medicine
基金
国家中医药管理局刘复兴传承工作室建设项目
