摘要
目的探讨小鼠骨髓间充质干细胞(BMSCs)亚群SCA-1^+/CD45^+/CD31^+抗凋亡的分子基础。方法以小鼠心脏干细胞表面分化抗原检测BMSCs后以CD45、CD31为标准分选得到4个亚群。体外将4个亚群采用无血清低氧(5%)培养,流式细胞术检测各组的凋亡率。分别将细胞注入心梗48 h小鼠体内于注射后48 h完成心功能测定。可见SCA-1^+/CD45^+/CD31^+组抗凋亡能力最强,心脏彩超提示心功能恢复亦优于其他各组。采用基因芯片完成Agilent小鼠全基因4×44K芯片从分子水平对SCA-1^+/CD45^+/CD31^+亚群抗凋亡能力进行探讨。结果将SCA-1^+/CD45^+/CD31^+与其他组有关于凋亡基因进行聚类分析及Network结果共同比较可见两者无交集,以上述差异基因4倍表达为标准,因而以Network结果为主。可见NFk B1、RB1、E2F1为感兴趣基因。结论小鼠BMSCs为一多克隆的细胞群体。SCA-1^+/CD45^+/CD31^+抗凋亡及改善心功能方面优于其他亚群;其分子机制在于NFk B1、RB1、E2F1基因表达较其他亚组多见。
Objective To investigate the molecular basis of the anti-apoptosis CD31^＋ subset of mice bone marrow mesenehymal stem cells （BMSCs）. Methods effects of the SCA-1^＋/ CD45^＋/ After the mice cardiac stem cell surface differentiation antigen was used to detect the BMSCs, four subsets were obtained, with CD45, CD31 as the sorting standard. These four groups were grown in vitro in serum-free cultures under hypoxia （5%）. The apoptosis rate of each group was measured by flow cytometry. The cell cultures were injected into mice with 48 h myocardial infarction to determine the cardiac function after another 48 h. Among the four groups, the SCA-1^＋/ CD45^＋/ CD31^＋ subset demonstrated the highest anti-apoptotic effect. Color sonography showed that the recovery of cardiac function in the SCA-1^＋/ CD45^＋/ CD31^＋ subset was relatively better than that in the other groups. An agilent whole mice genome 4 × 44K chip was used to study the anti-apoptosis effects of the SCA-1^＋/CD45^＋/CD31^＋ sub-set at the molecular level. Results Clustering analysis and network of the apoptosis genes was performed on the SCA-1^＋/CD45^＋/CD31^＋ subset against the other subsets. Results of network and Clustering analysis showed the absence of an intersection between groups. A fourfold difference in the expression of the above mentioned genes were set as the standard for differential expression based on the network results. NFkB1, RB1, and E2F1 were i- dentified as the genes of interest. Conclusion BMSCs belong to a polyclonal cell population. The SCA-1^＋/ CD45^＋/CD31^＋ subset is relatively better than the other subsets in terms of its anti-apoptosis effects and improve- ment in cardiac function. The molecular mechanism of SCA-1^＋/ CD45^＋/ CD31^＋ activity may involve NFkB1, RB1, and E2F1 because the expression is more common in the SCA-1^＋/CD45^＋/CD31^＋ subset than in the other subsets.
出处
《安徽医科大学学报》
CAS
北大核心
2015年第12期1757-1761,共5页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:81460073)
云南省科技厅-昆明医科大学应用基础研究联合专项(编号:2014FB089)
