摘要
研究大果沙棘总黄酮的体外抗氧化和抑菌活性。采用DPPH和ABTS自由基清除率检测大果沙棘总黄酮体外抗氧化活性;通过测定抑菌圈直径和最低抑菌浓度检测大果沙棘总黄酮的抑菌作用。100μg/m L的大果沙棘总黄酮对DPPH和ABTS自由基的清除率分别为65.1%和92.3%,接近芦丁和儿茶素对照品;抑菌试验结果显示:0.125 mg/m L^1.0 mg/m L的大果沙棘总黄酮对大肠杆菌、枯草芽孢杆菌和金黄色葡萄球菌有显著的抑菌效果。
The study was purported to research on the antioxidant activities in vitro and antibacterial activities of seabuckthorn flavonoids. The capacities of eliminating 1,1-dipheny1-2-picrylhydra-zyl（DPPH·） and the2,2＇-amino-di（2-ethyl-benzothiazoline sulphonic acid-6）ammonium salt（ABTS＋） were assayed compared with the standard sample of rutin and catechin controls. The antibacterial activities were studied through the detemination of the antibacterial circle diameter and minimum inhibitory concentration. The seabuckthorn flavonoids in 100 μg/m L had a capacities of eliminating DPPH· and ABTS＋ at the percentages of 65.1 % and92.3 %, respectively, approximate to the rutin and catechin at the same concentration and the antibacterial studies showed strong antibacterial effect against Escherichia coli, Staphylococcus aureus and Bacillus subtilis.
出处
《食品研究与开发》
CAS
北大核心
2015年第19期12-15,共4页
Food Research and Development
基金
黑龙江省教育厅项目(12521593)
关键词
大果沙棘总黄酮
抗氧化
抑菌活性
seabuckthorn flavonoids
antioxidation
antibacterial
