摘要
建立蔬菜中20种氨基甲酸酯类化合物高效液相色谱-静电场轨道阱高分辨质谱分析法。样品经乙腈提取,Qu ECh ERS方法对样品净化后,采用高效液相色谱-静电场轨道阱高分辨质谱法对样品中的目标化合物进行定性分析,外标法定量确证。结果表明:20种氨基甲酸酯类农药在0.005μg/m L^0.1μg/m L范围内线性关系均良好,R2均大于0.99,且所有农药定量限(LOQ)(S/N=10)均低于国际限量要求;在0.01、0.02 mg/kg和0.04 mg/kg的添加水平下,蔬菜中20种氨基甲酸酯类农药的平均回收率介于60%~120%之间,相对标准偏差(RSD)不大于5.0%。该方法较为简单,只需全扫描并提取精确质量数即可进行准确定量,受基质干扰小,能很好的满足我国进出口蔬菜中20种农药限量的严格要求,可以用于蔬菜基质中此20种氨基甲酸酯类农药残留的检测。
A method with the High Resolution Benchtop Oritrap Mass Spectrometer was established to rapid screen and identify accurate Mass of 20 carbamate pesticides in vegetables. The samples were extracted by acetonitrile and purified by the Qu ECh ERS, then qualitatively analyzed with the LC-OBMS and quantitatively analyzed with the external standard method. The results indicated the linearity of the method was good between0.005 μg/mL and 0.1 μg/mL, and all the limits of quantities（LOQ） of this method can meet the international standard. Meanwhile, the average percent recoveries of the twenty pesticides ranged from 60 % to 120 %, and the relative standard deviations（RSDS） were all less than 5.0 %, at three different addition levels（0.01, 0.02 and 0.04 μg/mL）. This simple method, which can exactly quantitative analyze just by full scaning and extracting exact mass number, and can overcome the matrix-effect influence, can be used for the detection of the 20 carbamate pesticides in vegetables, perfectly satisfying the pesticide residues limit requirements.
出处
《食品研究与开发》
CAS
北大核心
2015年第17期123-128,193,共7页
Food Research and Development
基金
山东出入境检验检疫局项目(SK201284)
关键词
静电场轨道阱
高分辨质谱
蔬菜
20种氨基甲酸酯
orbitrap
high resolution mass spectrometer
vegetables
20 carbamate pesticides
