摘要
目的:研究姜黄素对痤疮丙酸杆菌诱导的THP-1细胞分泌TNF-α和IL-8的影响,探讨姜黄素调控痤疮丙酸杆菌诱导的炎症反应的作用机制。方法:CCK8法检测姜黄素对THP-1细胞活力的影响;ELISA法检测上清中TNF-α和IL-8表达;实时定量PCR和Western blot检测TLR2及NF-κB p65表达。结果:姜黄素在5、10及15μmol/L浓度培养12 h后,对THP-1细胞活性无明显影响;姜黄素在10和15μmol/L浓度时,能抑制痤疮丙酸杆菌诱导的TNF-α和IL-8表达;姜黄素能抑制TLR2、NF-κB p65及P-NF-κB p65表达。结论:姜黄素能抑制痤疮丙酸杆菌诱导的THP-1细胞分泌炎症因子,其机制可能与抑制TLR2/NF-κB信号通路有关。
Objective To investigate the anti-inflammatory mechanism by which curcumin affects TNF-αand IL-8 production in Propionibactierium acnes-induced THP-1 cells. Methods THP-1 cell viability was determined by CCK8 assay. The productions of TNF-α and IL-8 were detected by ELISA assay. Total RNA and proteins were collected for real-time PCR and Western blot analysis, respectively. Results Curcumin didn′t significantly affect the cell viability at 12 h. It decreased Propionibactierium acnes-induced productions of TNF-αand IL-8 in THP-1 cells. Moreover, it decreased TLR2, NF-κB p65, and P-NF-κB p65 expressions in THP-1 cells. Conclusions Curcumin may reduce TNF-α and IL-8 expressions in Propionibactierium acnes-induced THP-1 cells by inhibiting TLR2/NF-κB signaling pathway.
出处
《实用医学杂志》
CAS
北大核心
2015年第20期3295-3297,共3页
The Journal of Practical Medicine
基金
国家自然科学基金(编号:81301341)
