摘要
目的观察自拟柴郁寄生汤对高催乳素血症(HPRL)模型大鼠卵巢组织PRL/PRLR-JAK2-STAT5信号传导通路的影响及其改善卵巢功能的机制。方法 Wistar大鼠60只皮下注射多巴胺受体阻断剂灭吐灵造成高催乳素血症模型。造模成功后按拉丁方法随机分为模型组、溴隐亭组、大剂量组、中剂量组、小剂量组各10只,并设正常组10只。中药高剂量组柴郁寄生汤剂量为18 g·kg^(1)·d^(1),中剂量组为9 g·kg^(1)·d^(1),低剂量组为4.5 g·kg^(1)·d^(1)。各药物组每只大鼠每次灌胃量均为2 ml。溴隐亭组给溴隐亭2 mg·kg^(1)·d^(1);模型组及正常组依据体质量予等容量蒸馏水灌胃;各组每日给药1次,连续给药30 d。治疗结束后将大鼠处死,量取大鼠卵巢体积;检测血清雌二醇(E_2)、促黄体生成素(FSH);通过Western blot及PCR法检测卵巢STAT、PRLR表达水平,以观察高催乳素血症环境对PRL/PRLR-JAK2/STAT5信号传导通路及卵巢功能的影响,并探讨其可能机制。结果与正常组比较,模型组大鼠血清PRL水平明皿升高,E_2、FSH水平均明显下降(P<0.05);卵巢STAT、PRLR蛋白表达水平均明显降低(P<0.05)。与模型组比较,各治疗组血清PRL水平均明显下降,血清E_2、FSH水平及卵巢STAT、PRLR表达水平均明显升高。不同治疗组间比较,中药中剂量组大鼠血清E_2、FSH水平改善最显明,卵巢STAT、PRLR蛋白表达明显最强(P<0.05),而PRL水平差异均无统计学意义(P>0.05)。结论柴郁寄生汤治疗高催乳素血症对改善卵巢功能有良好疗效,其作用机制可能是调节卵巢PRL/PRLR-JAK2/STAT5信号传导通路相关因子的表达,进而改善E_2、FSH等生殖激素水平。
Objective To observe the effect of Chaiyujisheng decoction on hyperprolactinemia（hPRL） model rat ovarian tissue PRL/PRLR-JAK2-STAT5 signaling pathway and improvement o（the ovarian function mechanism.Methods Wistar rats were injected with dopamine receptor blockers to cause a high Level ol prolactin（60 rats）.The model group was randomly divided into model group,bromocriptine group,large dose group,middle dose group,small dose group,and the normal group with 10 rats in each group.Traditional Chinese medicine high dose group＇s Chaiyujisheng decoction＇s amount was 18 g·kg^-1·d^-1,medium dose group was 9 g·kg^-1·d^-1,and low dose group was 4.5 g·kg^-1·d^-1.Each lime,the amount of each rat were fed with 2 ml.Bromocriptine group were given bromocriptine 2 mg·kg^-1·d^-1;model group and normal group according to body mass were given equal volume of distilled water by gavage.Each group administered once daily,continuous administration of 30 d.At the end of treatment,rats were sacrificed,rat ovarian volume were measured;detected serum estradiol（E_2）,luteinizing generated hormone（ESH）,through the method of Western blot and PCR to detect ovarian＇s STAT and PRLR expression level to observe the effects of hyperprolactinemia environment on PRL/PRLR-JAK2-STAT5 signaling pathway and ovarian function,and to explore its possible mechanism.Results Compared with the normal group,model group rats＇ serum PRE level increased significantly,E_2,ESH levels were significantly decreased（P 0.05）;ovarian＇s STAT and PRLR protein expression level were significantly decreased（P 0.05）.Compared with model group,in the treatment group,the serum PRL levels were significantly decreased and serum E_2,FSH levels and ovarian＇s STAT and PRLR expression levels were significantly increased.Between the different treatment groups,middle dose of traditional Chinese medicine group rats＇ serum E_2 and FSH levels improvement were the most obviously,and ovarian＇s STAT and PRLR protein expression was the highest（P 0.05）,and differences in the level of PRL had no statistical significance（P 0.05）.Conclusion It demonstrated that the Chaiyujisheng decoction could improve ovarian function in hyperprolactinemia,its mechanism maybe due to regulate the expression of ovarian PRL/PRLR-JAK2-STAT5 signaling pathway related factors,so as to improve E_2,FSH＇s level.
出处
《疑难病杂志》
CAS
2015年第11期1180-1185,共6页
Chinese Journal of Difficult and Complicated Cases
基金
北京中医药大学自主选题资助项目(No.2013-JYBZZ-JS-161)
关键词
高催乳素血症
卵巢功能下降
PRLR
STAT5
信号传导通路
大鼠
Hyperprolactinemia
Chaiyujisheng decoction
Ovarian function decline
PRLR
STAT5
Signal transduction pathway
Rats
