摘要
以"青芋2号"茎段为试验材料,考察了不同培养基对菊芋愈伤组织诱导、丛生芽诱导及生根培养的影响,并利用已优化的SSR-PCR反应体系对不同继代次数的菊芋试管苗进行遗传稳定性分析。结果表明:适宜菊芋离体增殖的培养基植物生长激素调节物质配比为MS+1mg/L6-BA+0.5mg/L NAA,适合菊芋生根培养基最佳配比为1/2MS+0.1mg/L IBA。从继代8次的组培苗中分析其遗传的变化,在检测出的50个条带中,个体之间未发生变异,生物学性状上亦未发现明显差异。试验表明在多次继代培养过程后,菊芋的遗传物质未发生明显变化,为今后菊芋快繁及离体保存等提供了技术参考。
Taking ‘QingYu 2＇stem segment explants as materials,the effect of different media on jerusalem artichoke callus induction,shoot induction and rooting culture was studied.The genetic stability of subculture jerusalem artichoke plantlets with different generation were analyzed using optimized SSR-PCR reaction system.The results showed that suitable media for cultivating jerusalem artichoke plants in vitro was：MS＋1.00mg/L 6-BA＋0.50mg/L NAA,and 1/2MS＋0.10mg/L IBA was suitable for rooting.Analysis of genetic variation from the subculture 8times in tissue culture,no variation between individuals among the 50 detected bands,also no significant differences in the biological traits.The results indicated that genetic material was not significantly changed in jerusalem artichoke,and provided a technology platform for propagation of jerusalem artichoke in future.
出处
《北方园艺》
CAS
北大核心
2015年第20期91-94,共4页
Northern Horticulture
基金
青海省科技厅资助项目(2012-H-809)
