摘要
目的:优化番石榴叶挥发油提取工艺,考察抑菌活性并分析其化学成分,为番石榴叶开发提供实验依据。方法:采用水蒸气蒸馏法提取番石榴叶挥发油,正交试验优化提取工艺;采用纸片扩散法探究番石榴叶挥发油的抑菌活性;气相色谱-质谱联用法(GC-MS)分析其化学成分。结果:最佳工艺条件:粉碎程度为粉末(20目),料液比为1∶12,时间为5h;挥发油对枯草芽孢杆菌、金黄色葡萄球菌、铜绿假单胞菌及大肠杆菌均有较好的抑菌效果,其最小抑菌浓度均为50μL/m L;从番石榴叶挥发油中共鉴定出23种成分,占挥发油总量的99.33%,主要为烯、醇等成分,其中,广藿香烯为其主要成分,含量占54.97%。结论:番石榴叶挥发油具有较好的抑菌活性,成分明确,可作为番石榴叶开发和利用的依据。
Objective：To optimize the extraction technology of the volatile oil in Psidium guajava L.,investigateits antibacterial activity,and analyze itschemical components,in order toprovide experimental basis for development and utilization of Psidium guajavaL..Methods：The volatile oil in Psidium guajava L.was extracted by using steam distillation method,and the extraction process was optimized by using orthogonal test.The antibacterial activity of volatile oil in Psidium guajava L.was investigated by using disk diffusion method,and the chemical components were analyzed by using gas chromatography-mass spectrometry（GC-MS）.Results：The optimal technology conditions of extraction of volatile oil in Psidium guajava L.was as follows：powder was the optimal degree of comminution;the ratio of material to liquor was 1：12;time was 5 hours.Volatile oil had obvious inhibitory effect on bacillus subtilis,staphylococcus aureus,pseudomonas aeruginosa and colon bacillus,and the minimal inhibitory concentration（MIC） was 50μL/mL.There were 23 chemical components were indentified in volatile oil of Psidium guajava L.,which accounted for 99.33%of the total volatile oil.The main components were alkenes and alcohol,in which patchoulene accounted for 54.97%.Conclusion：The volatile oil in Psidium guajava L.with explicit componentshadobvious antibacterial activity,which could be served as the basis for development and utilization ofPsidium guajava L..
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2015年第10期3754-3757,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(No.81503206
No.81202926)~~
关键词
番石榴叶
挥发油
正交试验
抑菌活性
气相色谱-质谱
广藿香烯
成分分析
提取工艺
Psidium guajava L.
Volatile oil
Orthogonal test
Antibacterial activity
Gas chromatography-mass spectrography
Patchoulene
Componets analysis
Extraction technology
