摘要
目的:探讨不同分期的多发性骨髓瘤(MM)患者外周血淋巴细胞各亚群细胞比例与白细胞介素-6(IL-6)表达变化。方法:用流式细胞仪检测35例初治MM患者及10例对照者的外周血淋巴细胞亚群各细胞表达水平,用ELISA法检测IL-6水平。结果:与对照者相比,MM患者的CD4+、CD4+/CD8+明显降低(P<0.01),均与MM分期严重程度呈负相关(r=-0.780,-0.832,P<0.01);MM患者的CD8+显著增加(P<0.01),与MM分期严重程度呈正相关(r=0.737,P<0.01)。MM患者的IL-6水平较对照者显著增加,与MM分期严重程度呈正相关(r=0.961,P<0.01)。结论:IL-6表达增加及T淋巴细胞亚群数目异常变化可能是MM患者免疫异常的机制之一,两者能作为观察MM患者病情严重程度的指标。
Objective：To investigate the changes of T lymphocytes subsels and expression of interleukin-6 （Il- 6） in peripheral blood of the patients with multiple myeloma （MM） in different stages. Method： The peripheral blood was collected from 35 newly diagnosed patients with MM and 10 normal controls. Flow cytometry was used to detect T lymphocyte subsets in peripheral blood, and enzyme-linked immunosorbent assay was used to determine the level of IL-6. Result：Compared with normal controls, the number of CD4＋ and the ratio of CD4＋/CD8＋ in MM patients decreased significantly （P〈0.01）,and were negatively correlated with the degree of MM （r=-0. 780, -0. 832, P〈0.01） ;The number of CD8＋ increased significantly,and was positively related with the degree of MM （r=0. 737,P〈0. 01） ;The level of IL-6 increased significantly, and was positively related with the degree of MM （r= 0.961, P〈0.01）. Conclusion：Increase of IL-6 expression and abnormality of T lymphocyte subsets may be one of the mechanisms for cellular immun dysfunction in patients with MM, both of them can serve as indexes for observing the disease state.
出处
《临床血液学杂志》
CAS
2015年第5期767-768,773,共3页
Journal of Clinical Hematology
