摘要
本试验克隆了高粱HKT(high affi nity potassium transporter)基因家族3个成员Sb HKT1;4,Sb HKT1;5和Sb HKT2;1,并分析比较了这些基因在拟南芥中转运钠、钾离子的特性以及在抗旱方面的作用。结果表明,外界高浓度钠胁迫对各转基因植株的生长均有一定的影响,但这种抑制作用可在外源添加足量钾离子的情况下得到不同程度的缓解。此外,干旱胁迫下,转基因植株表现出优于突变体的抗旱性。这些结果表明,高粱HKT基因不仅在作物耐盐方面具有重要作用,同时在植物抗旱中也具有一定的功能。
Three sorghum high affinity potassium transporter genes, Sb HKT1;4, Sb HKT1;5 and Sb HKT2;1, were cloned and constitutively expressed in Arabidopsis hkt1-1 mutant, respectively, and the tolerance to salt and drought of the transgenic lines analyzed. Upon high Na＋ stress, the growth of all transgenic lines was inhibited, and this phenotype could be obviously alleviated when adequate K^＋ was added. After water stress, we found that transgenic lines were more tolerant to drought than athkt1-1 mutant line. These results indicated that Sb HKTs not only played a role in tolerance to salt stress, but also exerted significant function in the presence of drought-stress.
出处
《植物生理学报》
CAS
CSCD
北大核心
2015年第9期1513-1523,共11页
Plant Physiology Journal
基金
国家科技支撑计划(2013BAD22B01和2015BAD15B03)
青年科学基金项目(31301326)
关键词
高粱HKT基因
拟南芥
耐盐性
抗旱性
sorghum HKT
Arabidopsis
salt resistance
drought tolerance
