摘要
目的:优选晕瘫通脑片的提取工艺。方法:以葛根素提取量和浸膏得率的综合评分为评价指标,在单因素试验基础上,通过Box-Behnken效应面法考察浸泡时间、加水量、提取时间对提取工艺的影响。结果:优选得到晕瘫通脑片的最佳提取工艺为加水8.6倍量,浸泡63.8 min后提取128.4 min,提取两次。结论:通过Box-Behnken效应面法及总评"归一值"(OD)为评价系统优选的提取工艺稳定、简便易行、预测性好,可为晕瘫通脑片的工艺研究提供参考。
Objective： To optimize extraction process of Yuntan Tongnao tablets by Box-Behnken design-response surface methodology. Methods： On the basis of single factor tests, Box-Behnken experimental design was employed to investigate effects of the extraction time,water amount and the soaking time on puerarin and yield of the extract. Results： The optimized processing parameters were as follows： soaking time 63. 8 minutes,8. 6 times volume of water, extract time128. 4minutes,twice extraction. Conclusion： The optimized extraction process is proved to be stable, simple, good predictability by Box-Behnken design-response surface methodology and overall desirability,it provides a basis for technology research of Yuntan Tongnao tablets.
出处
《中国现代中药》
CAS
2015年第9期957-961,共5页
Modern Chinese Medicine
基金
湖南省中医药科研计划项目(2010053)
