摘要
耐酸耐高温α-淀粉酶能在高温与低p H下水解淀粉,是重要的新型工业酶制剂。概述了耐酸耐高温α-淀粉酶的热稳定性、p H稳定性及金属离子对其的影响,阐述了产酶菌种的主要来源现状,并重点对耐酸耐高温α-淀粉酶诱变育种的复合诱变、基因工程定点突变等菌种选育和古菌的相关研究进行了综述。
The acid -resistant and thermostable α - amylases is one of the important industrial enzyme preparation, which can hydrolyze starch under high temperature and low pH. The influence of thermal stability, pH stability and metal ions on the acid -resistant and thermostable α -amylases was outlined. The main strain resources were summarized. The research progress in strain improvement and archaea, including compound mutation of mutation breeding and gene engineering site -directed mutagenesis, was discussed.
出处
《粮油食品科技》
2015年第5期101-105,共5页
Science and Technology of Cereals,Oils and Foods
基金
国家科技支撑计划项目(2013AA102101)
国家自然科学面上基金项目(31370147)
河南省高校科技创新团队支持计划(15IRTSTHN019)
河南省高校青年骨干教师资助计划(2013GGJS-077)
关键词
耐酸耐高温α-淀粉酶
酶学性质
菌种
选育
基因工程
acid - resistant thermostable α - amylase
enzymatic properties
strain
breeding
geneticengineering
